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Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody

Catalog #: MAB10348 Datasheet / COA / SDS
Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB10348-100
MAB10348-SP
Detection of PD-L1/B7-H1 in MDA-MB-231 human breast adenocarcinoma cell line by Flow Cytometry.
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Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects Human PD-L1/B7-H1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Atezolizumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu124
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human PD-L1/B7-H1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional flow cytometry test, 2.5 μg/mL of Human anti-human PD-L1/B7-H1 (Atezoliumab Biosimilar) Antibody(Catalog # MAB10348) will block the binding of Recombinant Human PD-1 His-tagged protein (Catalog # 8986-PD) to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PD-L1/B7-H1 antibody in MDA-MB-231 human breast adenocarcinoma cell line antibody by Flow Cytometry. View Larger

Detection of PD-L1/B7-H1 in MDA-MB-231 human breast adenocarcinoma cell line by Flow Cytometry. MDA-MB-231 human breast adenocarcinoma cell line was stained with Human Anti-Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10348, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (F0135). View our protocol for Staining Membrane-associated Proteins.

Blockade of Receptor-ligand Interaction View Larger

PD-1 binding to Human PD-L1/B7-H1-transfected HEK293 Human Cell Line is Blocked by Human PD-L1/B7-H1 Antibody. In a functional flow cytometry test, Recombinant Human PD-1 His-tagged protein (8986-PD, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 2.5 µg/mL of Human Anti-Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Monoclonal Antibody (Catalog # MAB10348) but not by (B) Human IgG Isotype Control (1-001-A). Ligand binding was detected by staining cells with Anti-His tag APC-conjugated Monoclonal Antibody (IC050A).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

Atezolizumab is a biosimilar directed against human PD-L1.

Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

Product Datasheets

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FAQs

  1. Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    • The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

  2. Is the Fc portion of Human PD-L1/B7-H1 (Research Grade Atezolizumab Biosimilar) Antibody, Catalog # MAB10348, inert, similar to what has been reported with the therapeutic Atezolizumab?

    • We have not performed any testing in house to confirm that the Fc portion is inert, but the sequence of MAB10348 is the same as the therapeutic Atezolizumab. 

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