Human PD-1 (Research Grade Pembrolizumab Biosimilar) Antibody

Catalog #: MAB11380 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB11380-100
MAB11380-SP

Detection of PD‑1 in HEK 293 cells by Flow Cytometry.

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Human PD-1 (Research Grade Pembrolizumab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects human PD-1 in direct ELISA. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Pembrolizumab. This product is for research use only.

Source

Monoclonal Human IgG4 Kappa Clone # Hu121

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK 293 cells transfected with eGFP and hPD-1 or irrelevant protein

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of PD‑1 in HEK 293 cells by Flow Cytometry. HEK 293 cells transfected with eGFP and hPD-1 (A) or irrelevant protein (B) were stained with Human Anti-Human PD-1 (Research Grade PembrolizumabBiosimilar) Monoclonal Antibody (Catalog # MAB11380) followed by Goat Anti-Human IgG Allophycocyanin (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: PD-1

Pembrolizumab is a humanized monoclonal IgG4 kappa PD-1 blocking antibody. PD-1 (programmed death receptor-1) is expressed on the surface of lymphocytes, and is also considered an immune checkpoint. The binding of the antibody with PD-1 interferes with the interaction with its ligands PD-L1 and PD-L2 preventing the inhibition of TCR-mediated T-cell proliferation. The inhibition of this PD-1 pathway results in enhanced immunosurveillance and anti-tumor immune response. The original therapeutic has been approved by the FDA for refractory melanoma, as well as other types of cancers such as non-small cell lung cancer, cervical cancer, head and neck cancer and Hodgkin's lymphoma.

Long Name

Programmed Death-1

Entrez Gene IDs

5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)

Alternate Names

CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2