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Human PD-1 Antibody

Catalog #: AF1086
65 Citations 7 Reviews Datasheet / COA / SDS
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AF1086
AF1086-SP
Detection of Human PD‑1 by Western Blot.
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Citations (65)
FAQs
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Reviews (7)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PD-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PD-1 in ELISAs and Western blots. In sandwich ELISAs, less than 2% cross-reactivity with recombinant mouse PD‑1 and less than 0.2% cross-reactivity with recombinant human (rh) CD28, rhICOS, and rhCTLA‑4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PD-1
Leu25-Gln167
Accession # Q8IX89
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5-2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 3-12 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of Recombinant Human B7-H1 Fc Chimera (Catalog # 156-B7) to immobilized Recombinant Human PD-1 Fc Chimera (Catalog # 1086-PD) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Dual RNAscope ISH-IHC
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human tonsil

Human PD-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human PD‑1 Biotinylated Antibody (Catalog # BAF1086)

Standard: Recombinant Human PD-1 Fc Chimera Protein, CF (Catalog # 1086-PD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PD‑1 antibody by Western Blot. View Larger

Detection of Human PD‑1 by Western Blot. Western blot shows lysate of human thymus tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD-1 at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human PD-1 antibody by Western Blot. View Larger

Detection of Human PD‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human PD-1. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for PD-1 at approximately 40-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of PD-1 antibody in Human PBMCs treated with PHA antibody by Flow Cytometry. View Larger

Detection of PD‑1 in Human PBMCs treated with PHA by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 5 µg/mL PHA overnight were stained with Goat Anti-Human PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry PD-1 antibody in Human Lymph Node by Immunohistochemistry (IHC-P). View Larger

PD‑1 in Human Lymph Node. PD-1 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human PD-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1086) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Detection of Human PD-1 by Immunohistochemistry View Larger

Detection of Human PD-1 by Immunohistochemistry PD-L1, PD-L2, PD-1, CD8, and CD4 expression in p16-positive and p16-negative HNSCC. PD-L1, PD-L2, PD-1, CD8, and CD4 expression was assessed in tumor biopsy tissue from five p16-positive and four p16-negative HNSCC patients using immuno-histochemistry (details in Methods). Representative staining (scale bars, 100 μm) and cumulative data of marker expression (grading scale PD-L1/PD-L2: 1, low; 2, moderate; 3 high expression; grading scale PD-1, CD8, CD4: 1, <50 cells/field; 2, 50–150 cells/field; 3, >150 cells/field). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31379843), licensed under a CC-BY license. Not internally tested by R&D Systems.

ELISA Detection of Human PD-1 by ELISA View Larger

Detection of Human PD-1 by ELISA Modulation of soluble co-signaling molecules in kidney-transplanted patients over time.The levels of the soluble co-signaling molecules CD30, CD40, CD137, CD40L, PD-1 and PD-L1 were assayed by ELISA in serum samples of healthy controls (n = 25) and kidney-transplanted patients (n = 59) obtained at different times: just before transplantation, and 15 days, 3 months and 1 year after transplantation. Data are shown as box-plots, in which the horizontal line within each box represents the median, the bottom and top of each box represent the 25th and 75th percentiles, the bars represent the 10th and 90th percentiles and circles indicate outliers. Unpaired and paired Wilcoxon tests were used to compare distributions between independent and dependent groups, respectively. * indicates statistically significant differences between healthy controls and kidney-transplanted patient samples, and † indicates statistically significant differences between patients samples obtained at different pre- and post-transplantation times. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25478957), licensed under a CC-BY license. Not internally tested by R&D Systems.

In-situ Hybridization View Larger

Detection of PD‑1 in Human Tonsil. Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for PD1 mRNA (ACD RNAScope Probe, catalog #602021; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human PD1 polyclonal antibody (R&D Systems catalog # AF1086) at 1ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cell surface.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-1

Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Members of the CD28/CTLA-4 family have been shown to either promote T cell activation (CD28 and ICOS) or down-regulate T cell activation (CTLA-4 and PD-1) (2). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. In vitro, ligation of PD-1 inhibits TCR-mediated T-cell proliferation and production of IL-1, IL-4, IL-10, and IFN-gamma. In addition, PD-1 ligation also inhibits BCR mediated signaling. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases (2, 3).

Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2 (also known as B7-DC), have been identified as PD-1 ligands. Unlike other B7 family proteins, both
PD‑L1 and PD-L2 are expressed in a wide variety of normal tissues including heart, placenta, and activated spleens (4). The wide expression of PD-L1 and PD-L2 and the inhibitor effects on PD-1 ligation indicate that PD-1 might be involved in the regulation of peripheral tolerance and may help prevent autoimmune diseases (2).

The human PD-1 gene encodes a 288 amino acid (aa) protein with a putative 20 aa signal peptide, a 148 aa extracellular region with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immuno-receptor
tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 60% aa sequence identity (4).

References
  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Nishimura, H. and T. Honjo (2001) Trends in Immunol. 22:265.
  3. Latchman, Y. et al. (2001) Nature Immun. 2:261.
  4. Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.
Long Name
Programmed Death-1
Entrez Gene IDs
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2

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Citations for Human PD-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

65 Citations: Showing 1 - 10
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  1. PD-L1/PD-1 expression and tumor-infiltrating lymphocytes in conjunctival melanoma
    Authors: Jinfeng Cao, Niels J. Brouwer, Kate E. Richards, Marina Marinkovic, Sjoerd van Duinen, Daan Hurkmans et al.
    Oncotarget
  2. Characterization of rectal, proximal and distal colon cancers based on clinicopathological, molecular and protein profiles
    Authors: P Minoo, I Zlobec, M Peterson, L Terracciano, A Lugli
    International Journal of Oncology
  3. TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2
    Authors: Paramita Baruah, Jessica Bullenkamp, Philip O. G. Wilson, Michael Lee, Juan Carlos Kaski, Ingrid E. Dumitriu
    Frontiers in Immunology
  4. Development and Fit-for-Purpose Validation of a Soluble Human Programmed Death-1 Protein Assay
    Authors: Yan G. Ni, Xiling Yuan, John A. Newitt, Jon E. Peterson, Carol R. Gleason, Jonathan Haulenbeek et al.
    The AAPS Journal
  5. Gut germinal center regeneration and enhanced antiviral immunity by mesenchymal stem/stromal cells in SIV infection
    Authors: Mariana G. Weber, Chara J. Walters-Laird, Amir Kol, Clarissa Santos Rocha, Lauren A. Hirao, Abigail Mende et al.
    JCI Insight
  6. Programmed Cell Death Ligand 1 Expression Is an Independent Prognostic Factor in Colorectal Cancer
    Authors: TUMENJIN ENKHBAT, MASAAKI NISHI, CHIE TAKASU, KOZO YOSHIKAWA, HIGASHIJIMA JUN, TAKUYA TOKUNAGA et al.
    Anticancer Research
  7. The Impact of Indoleamine 2,3-dioxygenase (IDO) Expression on Stage III Gastric Cancer
    Authors: MASAAKI NISHI, KOZO YOSHIKAWA, JUN HIGASHIJIMA, TAKUYA TOKUNAGA, HIDEYA KASHIHARA, CHIE TAKASU et al.
    Anticancer Research
  8. Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology
    Authors: Octavian Bucur, Feifei Fu, Mike Calderon, Geetha H. Mylvaganam, Ngoc L. Ly, Jimmy Day et al.
    Nature Protocols
  9. Mucosa-associated invariant T cells link intestinal immunity with antibacterial immune defects in alcoholic liver disease
    Authors: Antonio Riva, Vishal Patel, Ayako Kurioka, Hannah C Jeffery, Gavin Wright, Sarah Tarff et al.
    Gut
  10. Rapid and Long-term Response of Pulmonary Pleomorphic Carcinoma to Nivolumab
    Authors: Satoru Senoo, Takashi Ninomiya, Go Makimoto, Kazuya Nishii, Hirohisa Kano, Hiromi Watanabe et al.
    Internal Medicine
  11. Programmed cell death protein 1 expression is an independent prognostic factor in gastric cancer after curative resection
    Authors: Shohei Eto, Kozo Yoshikawa, Masaaki Nishi, Jun Higashijima, Takuya Tokunaga, Toshihiro Nakao et al.
    Gastric Cancer
  12. Programmed death-1–induced interleukin-10 production by monocytes impairs CD4+ T cell activation during HIV infection
    Authors: Elias A Said, Franck P Dupuy, Lydie Trautmann, Yuwei Zhang, Yu Shi, Mohamed El-Far et al.
    Nature Medicine

  13. Dimethyl Fumarate Targets MSK1, RSK1, 2 and IKK alpha / beta Kinases and Regulates NF-kappa B /p65 Activation in Psoriasis: A Demonstration of the Effect on Peripheral Blood Mononuclear Cells, Drawn from Two Patients with Severe Psoriasis Before and After Treatment with Dimethyl Fumarate
    Authors: Borbala Gesser, Mads K Rasmussen, Lars Iversen
    Psoriasis: Targets and Therapy
  14. Activation of the PD-1/PD-L1 immune checkpoint confers tumor cell chemoresistance associated with increased metastasis
    Authors: Madison Black, Ivraym B. Barsoum, Peter Truesdell, Tiziana Cotechini, Shannyn K. Macdonald-Goodfellow, Margaret Petroff et al.
    Oncotarget
  15. Comparison of Follicular Helper T-Cell Markers with the Expression of the Follicular Homing Marker CXCR5 in Peripheral T-Cell Lymphomas-A Reappraisal of Follicular Helper T-Cell Lymphomas
    Authors: Krenács, L;Krenács, D;Borbényi, Z;Tóth, E;Nagy, A;Piukovics, K;Bagdi, E;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  16. Modulation of immune checkpoint regulators in interferon ? induced urothelial carcinoma and activated T-lymphocyte cells by cytostatics
    Authors: H�nze, J;Schulte-Herbr�ggen, J;Hofmann, R;Hegele, A;
    Genes and immunity
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  17. Exosomes in malignant pleural effusion from lung cancer patients impaired the cytotoxicity of double-negative T cells
    Authors: J Wu, R Zhu, Z Wang, X Chen, T Xu, Y Liu, M Song, J Jiang, Q Ma, Z Chen, Y Liu, X Wang, M Zhang, M Huang, N Ji
    Translational Oncology, 2022-10-14;27(0):101564.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Escherichia coli-specific CXCL13-producing TFH are associated with clinical efficacy of neoadjuvant PD-1 blockade against muscle-invasive bladder cancer
    Authors: AG Goubet, L Lordello, C Alves Cost, I Peguillet, M Gazzano, MD Mbogning-F, C Thelemaque, C Lebacle, C Thibault, F Audenet, G Pignot, G Gravis, C Helissey, L Campedel, M Roupret, E Xylinas, I Ouzaid, A Dubuisson, M Mazzenga, C Flament, P Ly, V Marty, N Signolle, A Sauvat, T Sbarrato, M Filahi, C Davin, G Haddad, J Bou Khalil, C Bleriot, FX Danlos, G Dunsmore, K Mulder, A Silvin, T Raoult, B Archambaud, S Belhechmi, I Gomperts B, N Cayet, M Moya-Nilge, A Mallet, R Daillere, E Rouleau, C Radulescu, Y Allory, J Fieschi, M Rouanne, F Ginhoux, G Le Teuff, L Derosa, A Marabelle, J VAN Dorp, N VAN Dijk, MS van der He, B Besse, F Andre, M Merad, G Kroemer, JY Scoazec, L Zitvogel, Y Loriot
    Cancer Discovery, 2022-10-05;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis
    Authors: S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood
    Nature Communications, 2022-06-03;13(1):3121.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Additive Intralesional Interleukin-2 Improves Progression-Free Survival in a Distinct Subgroup of Melanoma Patients with Prior Progression under Immunotherapy
    Authors: D Rafei-Sham, S Lehr, M Behrens, F Meiss
    Cancers, 2022-01-21;14(3):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  21. The role of the immunoescape in colorectal cancer liver metastasis
    Authors: C Takasu, S Yamashita, Y Morine, K Yoshikawa, T Tokunaga, M Nishi, H Kashihara, T Yoshimoto, M Shimada
    PLoS ONE, 2021-11-19;16(11):e0259940.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. CD4+ T Cells Are Dispensable for Induction of Broad Heterologous HIV Neutralizing Antibodies in Rhesus Macaques
    Authors: Sarkar S, Spencer DA, Barnette P et al.
    Frontiers in Immunology
  23. Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling
    Authors: C Glorieux, X Xia, YQ He, Y Hu, K Cremer, A Robert, J Liu, F Wang, J Ling, PJ Chiao, P Huang
    Redox Biology, 2020-11-03;38(0):101780.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Impact of sidedness of colorectal cancer on tumor immunity
    Authors: C Takasu, M Nishi, K Yoshikawa, T Tokunaga, H Kashihara, T Yoshimoto, M Shimada
    PLoS ONE, 2020-10-12;15(10):e0240408.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  25. The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/&beta-catenin signaling pathway
    Authors: R Deng, C Zuo, Y Li, B Xue, Z Xun, Y Guo, X Wang, Y Xu, R Tian, S Chen, Q Liu, J Chen, J Wang, X Huang, H Li, M Guo, X Wang, M Yang, Z Wu, J Wang, J Ma, J Hu, G Li, S Tang, Z Tu, H Ji, H Zhu
    Cell. Mol. Immunol., 2020-09-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  26. Concordance of PD-1 and PD-L1 (B7-H1) in paired primary and metastatic clear cell renal cell carcinoma
    Authors: JE Eckel-Pass, TH Ho, DJ Serie, JC Cheville, R Houston Th, BA Costello, H Dong, ED Kwon, BC Leibovich, AS Parker
    Cancer Med, 2019-12-12;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  27. Mechanisms utilized by feline adipose-derived mesenchymal stem cells to inhibit T lymphocyte proliferation
    Authors: N Taechangam, SS Iyer, NJ Walker, B Arzi, DL Borjesson
    Stem Cell Res Ther, 2019-06-25;10(1):188.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade
    Authors: S Yang, J Wei, YH Cui, G Park, P Shah, Y Deng, AE Aplin, Z Lu, S Hwang, C He, YY He
    Nat Commun, 2019-06-25;10(1):2782.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  29. Low levels of SIV-specific CD8+ T cells in germinal centers characterizes acute SIV infection
    Authors: S Li, JM Folkvord, KJ Kovacs, RK Wagstaff, G Mwakalundw, AK Rendahl, EG Rakasz, E Connick, PJ Skinner
    PLoS Pathog., 2019-03-21;15(3):e1007311.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  30. Tumor-derived exosomal HMGB1 promotes esophageal squamous cell carcinoma progression through inducing PD1+ TAM expansion
    Authors: B Li, TN Song, FR Wang, C Yin, Z Li, JP Lin, YQ Meng, HM Feng, T Jing
    Oncogenesis, 2019-02-22;8(3):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Analysis of expression of the PD-1/PD-L1 immune checkpoint system and its prognostic impact in gastroenteropancreatic neuroendocrine tumors
    Authors: M Sampedro-N, A Serrano-So, M Adrados, JM Cameselle-, C Blanco-Car, JM Cabezas-Ag, R Martínez-H, E Martín-Pér, JL Muñoz de N, JÁ Díaz, R García-Cen, J Caneiro-Gó, I Abdulkader, R González-A, M Marazuela
    Sci Rep, 2018-12-13;8(1):17812.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  32. Tumor-derived exosomes induce PD1+ macrophage population in human gastric cancer that promotes disease progression
    Authors: F Wang, B Li, Y Wei, Y Zhao, L Wang, P Zhang, J Yang, W He, H Chen, Z Jiao, Y Li
    Oncogenesis, 2018-05-25;7(5):41.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC
  33. Altered Ratio of T Follicular Helper Cells to T Follicular Regulatory Cells Correlates with Autoreactive Antibody Response in Simian Immunodeficiency Virus-Infected Rhesus Macaques
    Authors: W Fan, AJ Demers, Y Wan, Q Li
    J. Immunol., 2018-04-02;0(0):.
    Species: Primate
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Associations of Simian Immunodeficiency Virus (SIV)-Specific Follicular CD8+T Cells with Other Follicular T Cells Suggest Complex Contributions to SIV Viremia Control
    Authors: MA Rahman, KM McKinnon, TS Karpova, DA Ball, DJ Venzon, W Fan, G Kang, Q Li, M Robert-Gur
    J. Immunol., 2018-03-05;0(0):.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC
  35. Blockade of Tumor-Expressed PD-1 promotes lung cancer growth
    Authors: S Du, N McCall, K Park, Q Guan, P Fontina, A Ertel, T Zhan, AP Dicker, B Lu
    Oncoimmunology, 2018-01-29;7(4):e1408747.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  36. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination
    Authors: D Amodio, N Cotugno, G Macchiarul, S Rocca, Y Dimopoulos, MR Castrucci, R De Vito, FM Tucci, AB McDermott, S Narpala, P Rossi, RA Koup, P Palma, C Petrovas
    J. Immunol., 2017-12-13;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  37. Increased expression of programmed cell death protein 1 on NK cells inhibits NK-cell-mediated anti-tumor function and indicates poor prognosis in digestive cancers
    Authors: Y Liu, Y Cheng, Y Xu, Z Wang, X Du, C Li, J Peng, L Gao, X Liang, C Ma
    Oncogene, 2017-07-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay, Neutralization
  38. Immune Cell Dynamics in Rhesus Macaques Infected with a Brazilian Strain of Zika Virus
    Authors: ELV Silveira, KA Rogers, S Gumber, P Amancha, P Xiao, SM Woollard, SN Byrareddy, MM Teixeira, F Villinger
    J. Immunol., 2017-06-30;0(0):.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  39. Particulate Array of Well-Ordered HIV Clade C Env Trimers Elicits Neutralizing Antibodies that Display a Unique V2 Cap Approach
    Authors: P Martinez-M, K Tran, J Guenaga, G Lindgren, M Àdori, Y Feng, GE Phad, N Vázquez Be, S Bale, J Ingale, V Dubrovskay, S O'Dell, L Pramanik, M Spångberg, M Corcoran, K Loré, JR Mascola, RT Wyatt, GB Karlsson H
    Immunity, 2017-05-16;46(5):804-817.e7.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC
  40. PD-L1, PD-L2 and PD-1 expression in metastatic melanoma: Correlation with tumor-infiltrating immune cells and clinical outcome
    Authors: Joseph M Obeid
    Oncoimmunology, 2016-09-20;5(11):e1235107.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  41. PD-1 Blockade with Pembrolizumab in Advanced Merkel-Cell Carcinoma
    Authors: PT Nghiem, S Bhatia, EJ Lipson, RR Kudchadkar, NJ Miller, L Annamalai, S Berry, EK Chartash, A Daud, SP Fling, PA Friedlande, HM Kluger, HE Kohrt, L Lundgren, K Margolin, A Mitchell, T Olencki, DM Pardoll, SA Reddy, EM Shantha, WH Sharfman, E Sharon, She
    N Engl J Med, 2016-04-19;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  42. Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques.
    Authors: Vargas-Inchaustegui D, Demers A, Shaw J, Kang G, Ball D, Tuero I, Musich T, Mohanram V, Demberg T, Karpova T, Li Q, Robert-Guroff M
    J Immunol, 2016-01-15;196(4):1700-10.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  43. Plasmacytoid dendritic cells promote HIV-1-induced group 3 innate lymphoid cell depletion.
    Authors: Zhang Z, Cheng L, Zhao J, Li G, Zhang L, Chen W, Nie W, Reszka-Blanco N, Wang F, Su L
    J Clin Invest, 2015-08-24;125(9):3692-703.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  44. Soluble co-signaling molecules predict long-term graft outcome in kidney-transplanted patients.
    Authors: Melendreras S, Martinez-Camblor P, Menendez A, Bravo-Mendoza C, Gonzalez-Vidal A, Coto E, Diaz-Corte C, Ruiz-Ortega M, Lopez-Larrea C, Suarez-Alvarez B
    PLoS ONE, 2014-12-05;9(12):e113396.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  45. Early lymphoid responses and germinal center formation correlate with lower viral load set points and better prognosis of simian immunodeficiency virus infection.
    Authors: Hong J, Amancha P, Rogers K, Courtney C, Havenar-Daughton C, Crotty S, Ansari A, Villinger F
    J Immunol, 2014-06-06;193(2):797-806.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  46. CD4 T follicular helper cell dynamics during SIV infection.
    J. Clin. Invest., 2012-08-27;122(9):3281-94.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  47. Modulation of T-cell activation by malignant melanoma initiating cells.
    Authors: Schatton T, Schutte U, Frank NY, Zhan Q, Hoerning A, Robles SC, Zhou J, Hodi FS, Spagnoli GC, Murphy GF, Frank MH
    Cancer Res., 2010-01-12;70(2):697-708.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  48. Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.
    Authors: Estes JD, Gordon SN, Zeng M, Chahroudi AM, Dunham RM, Staprans SI, Reilly CS, Silvestri G, Haase AT
    J. Immunol., 2008-05-15;180(10):6798-807.
    Species: Primate - Macaca mulatta (Rhesus Macaque), Primate - Cercocebus atys (Sooty Mangabey)
    Sample Types: Whole Tissue
    Applications: IHC
  49. Soluble PD-1 rescues the proliferative response of simian immunodeficiency virus-specific CD4 and CD8 T cells during chronic infection.
    Authors: Onlamoon N, Rogers K, Mayne AE, Pattanapanyasat K, Mori K, Villinger F, Ansari AA
    Immunology, 2008-02-05;124(2):277-93.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Neutralization
  50. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis.
    Authors: Wan B, Nie H, Liu A, Feng G, He D, Xu R, Zhang Q, Dong C, Zhang JZ
    J. Immunol., 2006-12-15;177(12):8844-50.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  51. Clinical Significance of PD-L1+ Exosomes in Plasma of Head and Neck Cancer Patients
    Authors: Marie-Nicole Theodoraki, Saigopalakrishna S. Yerneni, Thomas K. Hoffmann, William E. Gooding, Theresa L. Whiteside
    Clinical Cancer Research
  52. A B cell follicle sanctuary permits persistent productive SIV infection in elite controllers.
    Authors: Fukazawa Y, Lum R, Okoye AA et al.
    Nat Med
  53. Multiple Immunostainings with Different Epitope Retrievals—The FOLGAS Protocol
    Authors: Anna von Schoenfeld, Peter Bronsert, Michael Poc, Andrew Fuller, Andrew Filby, Stefan Kraft et al.
    International Journal of Molecular Sciences
  54. Differential Expression of Programmed Death-1 (PD-1) in Sézary Syndrome and Mycosis Fungoides
    Authors: Fatma Çetinözman, Patty M. Jansen, Maarten H. Vermeer, Rein Willemze
    Archives of Dermatology
  55. TIA-1 Cytotoxic Granule-Associated RNA Binding Protein Improves the Prognostic Performance of CD8 in Mismatch Repair-Proficient Colorectal Cancer
    Authors: Inti Zlobec, Eva Karamitopoulou, Luigi Terracciano, Salvatore Piscuoglio, Giandomenica Iezzi, Manuele Giuseppe Muraro et al.
    PLoS ONE
  56. The Intrahepatic Expression and Distribution of BTLA and its Ligand HVEM in patients with HBV-related acute-on-chronic liver failure
    Authors: Huan Xu, Dayan Cao, Guoning Guo, Zhihua Ruan, Yuzhang Wu, Yongwen Chen
    Diagnostic Pathology
  57. Cancer cell intrinsic TIM-3 induces glioblastoma progression
    Authors: Qing Guo, Shuai Shen, Gefei Guan, Chen Zhu, Cunyi Zou, Jingyuan Cao et al.
    iScience
  58. CD4+ T Cells Are Dispensable for Induction of Broad Heterologous HIV Neutralizing Antibodies in Rhesus Macaques
    Authors: Sarkar S, Spencer DA, Barnette P et al.
    Frontiers in Immunology
  59. The Possible Role of PD-1 Protein in Ganoderma lucidum-Mediated Immunomodulation and Cancer Treatment
    Authors: Gan Wang, Le Wang, Jianlong Zhou, Xiaoxin Xu
    Integr Cancer Ther
  60. Cellular and soluble immune checkpoint signaling forms PD-L1 and PD-1 in renal tumor tissue and in blood
    Authors: Corinna U. Keber, Marcus Derigs, Carolin Schultz, Moritz Wegner, Susanne Lingelbach, Viktoria Wischmann et al.
    Cancer Immunology, Immunotherapy
  61. PD-1 expression and clinical PD-1 blockade in B-cell lymphomas
    Authors: Zijun Y. Xu-Monette, Jianfeng Zhou, Ken H. Young
    Blood
  62. Anti‐PD‐L1/TGF‐ beta R fusion protein (SHR‐1701) overcomes disrupted lymphocyte recovery‐induced resistance to PD‐1/PD‐L1 inhibitors in lung cancer
    Authors: Bo Cheng, Kaikai Ding, Pengxiang Chen, Jianxiong Ji, Tao Luo, Xiaofan Guo et al.
    Cancer Communications
  63. Anti-PD1 ‘SHR-1210ʹ aberrantly targets pro-angiogenic receptors and this polyspecificity can be ablated by paratope refinement
    Authors: William J.J. Finlay, James E. Coleman, Jonathan S. Edwards, Kevin S. Johnson
    mAbs
  64. Tumor-endogenous PD-1 promotes cell proliferation and predicts poor survival in non-small cell lung cancer
    Authors: Fanyi Gan, Chuanfen Zhang, Liang Xia, Senyi Deng
    Translational Cancer Research
  65. Targeting the PD1/PD-L1 axis in melanoma: Biological rationale, clinical challenges and opportunities
    Authors: Barbara Merelli, Daniela Massi, Laura Cattaneo, Mario Mandalà
    Critical Reviews in Oncology/Hematology

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Human PD-1 Antibody
By Negar FIROOZI on 08/28/2023
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

Letivirus carrying human PD-1 was used to infect 293T cells. The membrane was exposed to a concentration of 1 µg/mL Human PD‑1 Monoclonal Antibody. PD‑1 band was identified at approximately 45 kDa.

Western Blot Human PD-1 Antibody AF1086
Human PD-1 Antibody
By Anonymous on 11/23/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human
Immunocytochemistry/Immunofluorescence Human PD-1 Antibody AF1086
Human PD-1 Antibody
By Anonymous on 07/23/2018
Application: WB Sample Tested: 293T human embryonic kidney cell line Species: Human

293T cells were infected by lentivirus overexpression of control or PD-1 for 24 and 48 h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1 um/ml Human PD-1 Antibody (AF1086). A specific band was detected for PD-1 at approximately 43 kDa. This experiment was conducted under reducing conditions

Western Blot Human PD-1 Antibody AF1086
Human PD-1 Antibody
By Casey Keyes on 03/07/2018
Application: ELISA Sample Tested: Human recombinant antibody Species: Human
Human PD‑1 Antibody
By Anonymous on 11/24/2017
Application: Simple Western Sample Tested: 2120Ep human embryonal carcinoma cell line Species: Human
Human PD-1 Antibody
By Anonymous on 09/29/2017
Application: ELISA Sample Tested: Human recombinant test antibody Species: Human
Human PD-1 Antibody
By Anonymous on 03/14/2017
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

10^6 Human PBMCs were probed with 0.5 ug of Goat anti-Human PD-1 antibody (red), or Goat Isotype Control IgG (black), followed by PE-conjugated anti-Goat secondary antibody.

Flow Cytometry Human PD-1 Antibody AF1086