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Human Olig 1, 2, 3 Antibody

Catalog #: MAB2230
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2230
MAB2230-SP
Olig 1, 2, 3 in Mouse Embryo.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Olig 1, 2, 3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Olig 1, 2, 3. In direct ELISAs, this antibody shows approximately 20% cross‑reactivity with recombinant human (rh) Olig2 and 50 - 100% cross‑reactivity with rhOlig3.
Source
Monoclonal Mouse IgG2A Clone # 257224
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Olig1
aa 1-272
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo.
Immunocytochemistry
8-25 µg/mL
Immersion fixed A172 human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Olig 1, 2, 3 in Mouse Embryo. Olig 1, 2, 3 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Mouse Anti-Human Olig 1, 2, 3 Monoclonal Antibody (Catalog # MAB2230) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in neurons in developing brain. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olig 1, 2, 3

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in neural tissues.

Long Name
Oligodendrocyte Lineage Gene
Alternate Names
bHLHb6; bHLHe21; Olig 1, 2, 3

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Citations for Human Olig 1, 2, 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Generating Cell Type-Specific Protein Signatures from Non-symptomatic and Diseased Tissues
    Authors: Jessica S. Sadick, Lorin A. Crawford, Harry C. Cramer, Christian Franck, Shane A. Liddelow, Eric M. Darling
    Annals of Biomedical Engineering
  2. Involvement of ER stress in dysmyelination of Pelizaeus-Merzbacher Disease with PLP1 missense mutations shown by iPSC-derived oligodendrocytes.
    Authors: Numasawa-Kuroiwa Y, Okada Y, Shibata S, Kishi N, Akamatsu W, Shoji M, Nakanishi A, Oyama M, Osaka H, Inoue K, Takahashi K, Yamanaka S, Kosaki K, Takahashi T, Okano H
    Stem Cell Reports, 2014-04-24;2(5):648-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Relevance of combinatorial profiles of intermediate filaments and transcription factors for glioma histogenesis.
    Authors: Colin C, Virard I, Baeza N, Tchoghandjian A, Fernandez C, Bouvier C, Calisti A, Tong S, Durbec P, Figarella-Branger D
    Neuropathol. Appl. Neurobiol., 2007-04-17;33(4):431-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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