Human NT-4 Propeptide Antibody Summary
Gln25-Arg80
Accession # P34130
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human NT‑4 Propeptide antibody by Western Blot. Detection of Human NT‑4 Propeptide antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/NT-4_AF3450_Western_Blot_5301.jpg)
Detection of Human NT‑4 Propeptide by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line, LNCaP human prostate cancer cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human NT-4 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3450) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NT-4 Propeptide at approximately 36 kDa (corresponent to proform). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NT-4
NT-4 Propeptide (neurotrophin-4/5) is a 27 kDa, secreted, glycosylated neuronal polypeptide that belongs to the NGF-beta family of neurotrophic factors. It is synthesized as a proform that contains a 9 kDa, 56 amino acid (aa) proregion (aa 25-80) and a 130 aa mature segment. The mature molecule forms both nondisulfide linked heterodimers (with NGF) and homodimers. It is unclear if 186 aa NT-4 Propeptide dimerizes, but this might be anticipated. Proneurotrophins are believed to activate NGFR and sortilin complexes and neurotrophin proregions regulate neurotrophin maturation. Over aa 25-80, human NT-4 Propeptide is 89% and 91% aa identical to mouse and canine NT-4 Propeptide, respectively.
Product Datasheets
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