Human NRAGE Antibody Summary
Met1-Glu778
Accession # Q9Y5V3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![NRAGE antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). NRAGE antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/NRAGE_MAB3835_Immunocytochemistry__Immunofluorescence_20174.jpg)
NRAGE in C2C12 Mouse Cell Line. NRAGE was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB3835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NRAGE
Neurotrophin receptor-interacting MAGE homolog (NRAGE, also known as MAGE-D1 or Dlxin-1) is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression and facilitates NGF-dependent apoptosis. Over amino acids 73‑254, human NRAGE shares 87% and 73% aa identity with mouse and rat NRAGE, respectively.
Product Datasheets
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