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Human NOV/CCN3 Antibody

Catalog #: AF1640
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1640
AF1640-SP
NOV/CCN3 in Human Kidney Cancer Tissue.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NOV/CCN3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NOV/CCN3 in ELISAs and Western blots. In sandwich ELISAs, approximately 7% cross-reactivity with recombinant mouse NOV/CCN3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human NOV/CCN3
Thr32-Met357
Accession # P48745
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human NOV/CCN3 (Catalog # 1640-NV)
Immunohistochemistry
5-15 µg/mL
See below

Human NOV/CCN3 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human NOV/CCN3 Biotinylated Antibody (Catalog # BAF1640)

Standard: Recombinant Human NOV/CCN3 Protein, CF (Catalog # 1640-NV)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry NOV/CCN3 antibody in Human Kidney Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

NOV/CCN3 in Human Kidney Cancer Tissue. NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NOV/CCN3

NOV, also called CCN3, is one of six CCN (CYR61/CTGF/NOV) secreted proteins which share a common multimodular organization (1-4). NOV/CCN3 contains an N‑terminal IGFBP domain that appears to be non-functional and a vWF type C and thrombospondin type I domain which mediate oligomerization and matrix interactions, respectively (1, 2). The C-terminal cysteine knot domain interacts with several partners, including the matrix protein fibulin 1C (5), Notch-1 (6), and CCN2, with which it may heterodimerize (2). NOV/CCN3 also interacts with the gap junction protein Connexin43 and mediates suppression of proliferation (7). It also binds the calcium binding protein S100A4 and promotes calcium channel activation (8). The 357 amino acid (aa), 44 kDa human NOV/CCN3 shares 80% aa identity with mouse, rat and dog NOV/CCN3, and 78% aa identity with cow NOV/CCN3. NOV/CCN3 also shows 38-50% aa identity with other family members including WISP proteins, except for WISP-2/CCN5 which lacks the cysteine knot (1). NOV/CCN3 is widely expressed developmentally, especially in muscle, endothelium, nervous system, adrenal cortex and chondrocytes (1-4). In transformed cells, a 32 kDa N-terminally truncated form lacks the signal sequence is localized to the nucleus. Truncation allows a C‑terminal nuclear localization sequence to be active (9). Nuclear NOV/CCN3 acts as a transcriptional repressor but promotes proliferation, presumably by interfering with growth control (9). Full length NOV/CCN3 is a secreted matricellular protein which inhibits cell growth. Interaction of NOV/CCN3 with integrins alpha v beta 3 and  alpha 5 beta 1 mediates endothelial cell adhesion, chemotaxis, and promotes angiogenesis (10, 11). Over-expression of NOV/CCN3 downregulates myogenic genes such as MyoD (12).

References
  1. Perbal, B. (2004) Lancet 363:62.
  2. Perbal, B. (2006) Cell Commun. Signal. 4:3.
  3. Martinerie, C. et al. (1994) Oncogene 9:2729.
  4. Snaith, M.R. et al. (1996) Genomics 38:425.
  5. Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.
  6. Sakamoto, K. et al. (2002) J. Biol. Chem. 277:29399.
  7. Fu, C.T. et al. (2004) J. Biol. Chem. 279:36943.
  8. Li, C.L. et al. (2002) Mol. Pathol. 55:250.
  9. Planque, N. et al. (2006) J. Cell. Biochem. 99:105.
  10. Lin, C.G. et al. (2003) J. Biol. Chem. 278:24200.
  11. Lin, C.G. et al. (2003) J. Biol. Chem. 280:8229.
  12. Calhabeu, F. et al. (2006) Exp. Cell. Res. 312:1876.
Long Name
Nephroblastoma Overexpressed Gene
Entrez Gene IDs
4856 (Human); 18133 (Mouse)
Alternate Names
CCN family member 3; CCN3; CCN3IGF-binding protein 9; IBP-9; IGFBP-9; IGFBP9novH; Insulin-like growth factor-binding protein 9; nephroblastoma overexpressed gene; Nephroblastoma-overexpressed gene protein homolog; NOV; NOVH; protein NOV homolog

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Citations for Human NOV/CCN3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. CCN3 is dynamically regulated by treatment and disease state in multiple sclerosis
    Authors: M Naughton, J Moffat, G Eleftheria, N de la Vega, A Young, J Falconer, K Hawkins, B Pearson, B Perbal, A Hogan, P Moynagh, S Loveless, NP Robertson, B Gran, R Kee, S Hughes, G McDonnell, O Howell, DC Fitzgerald
    J Neuroinflammation, 2020-11-22;17(1):349.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  2. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
    Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
    Breast Cancer Res., 2019-02-27;21(1):31.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC-P
  3. Renal Fibrosis mRNA Classifier: Validation in Experimental Lithium-Induced Interstitial Fibrosis in the Rat Kidney
    PLoS ONE, 2016-12-21;11(12):e0168240.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Matricellular protein CCN3 (NOV) regulates actin cytoskeleton reorganization.
    Authors: Sin WC, Tse M, Planque N, Perbal B, Lampe PD, Naus CC
    J. Biol. Chem., 2009-08-25;284(43):29935-44.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

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