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Human NGFI-B alpha /Nur77/NR4A1 Antibody

Catalog #: PP-H1648-00
3 Citations Datasheet / COA / SDS
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PP-H1648-00
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Citations (3)
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Human NGFI-B alpha /Nur77/NR4A1 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human NGFI-B alpha but does not recognize NGFI-B beta and gamma. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # H1648
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human NGFI-B alpha/Nur77/NR4A1
aa 2-249
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL with the appropriatesecondary reagents to detect human NGFI-B alpha.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Direct ELISA
This antibody can be used at 0.3 μg/mL with the appropriatesecondary reagents to detect human NGFI-B alpha.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: NGFI-B alpha/Nur77/NR4A1

Nerve growth factor inducible factor I-B alpha (NGFI-B alpha, Nur77, TR3, HMR; NR4A1) is a member of the Orphan Nuclear Receptor family. NGFI-B alpha is expressed in the brain, adrenals, thyroid, liver, testis, ovary, thymus, muscle, lung and prostate. Its expression is induced in response to various stress stimuli and growth factors. NGFI-B alpha is involved in mediating apoptotic signaling in thymocytes and tumor cells, as well as having a role in signaling in the hypothalamic-pituitary axis. RXR has been shown to be a partner for NGFI-B alpha.

Long Name
Nerve Growth Factor-induced Protein I-B alpha/Nuclear Receptor Subfamily 4, Group A, Member 1
Entrez Gene IDs
3164 (Human)
Alternate Names
Early response protein NAK1; GFRP1ST-59; growth factor-inducible nuclear protein N10; HMR; HMRNP10; hormone receptor; MGC9485; N10; NAK1; NAK-1; NGFIB alpha; NGFI-B alpha; NGFIB; NR4A1; Nuclear hormone receptor NUR/77; nuclear receptor subfamily 4 group A member 1; nuclear receptor subfamily 4, group A, member 1; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; steroid receptor TR3; Testicular receptor 3; TR3 orphan receptor; TR3

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Citations for Human NGFI-B alpha /Nur77/NR4A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Modulation of orphan nuclear receptor Nur77-mediated apoptotic pathway by acetylshikonin and analogues.
    Authors: Liu J, Zhou W, Li SS, Sun Z, Lin B, Lang YY, He JY, Cao X, Yan T, Wang L, Lu J, Han YH, Cao Y, Zhang XK, Zeng JZ
    Cancer Res., 2008-11-01;68(21):8871-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Nur77 converts phenotype of Bcl-B, an antiapoptotic protein expressed in plasma cells and myeloma.
    Authors: Luciano F, Krajewska M, Ortiz-Rubio P, Krajewski S, Zhai D, Faustin B, Bruey JM, Bailly-Maitre B, Lichtenstein A, Kolluri SK, Satterthwait AC, Zhang XK, Reed JC
    Blood, 2007-01-16;109(9):3849-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  3. NR4A1 regulates expression of immediate early genes, suppressing replication stress in cancer
    Authors: Guo H, Golczer G, Wittner BS Et al.
    Molecular cell

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