Human Neuronal Pentraxin 2 Antibody

Catalog #: MAB78162 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB78162-100
MAB78162-SP

Neuronal Pentraxin 2 in Human Pituitary (Anterior Lobe).

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Human Neuronal Pentraxin 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neuronal Pentraxin 2 in direct ELISAs.

Source

Monoclonal Mouse IgG2b Clone # 569720

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Neuronal Pentraxin 2 Monoclonal Antibody (Catalog # MAB78161).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

0.5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Neuronal Pentraxin 2 in Human Pituitary (Anterior Lobe). Neuronal Pentraxin 2 was detected in immersion fixed paraffin-embedded sections of human pituitary (anterior lobe) using Mouse Anti-Human Neuronal Pentraxin 2 Monoclonal Antibody (Catalog # MAB78162) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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Neuronal Pentraxin 2 in Human Pituitary. Neuronal Pentraxin 2 was detected in immersion fixed paraffin-embedded sections of human pituitary tissue using Mouse Anti-Human Neuronal Pentraxin 2 Monoclonal Antibody (Catalog # MAB78162) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

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Neuronal Pentraxin 2 in Human Pancreas. Neuronal Pentraxin 2 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Neuronal Pentraxin 2 Monoclonal Antibody (Catalog # MAB78162) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

ELISA

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Human Neuronal Pentraxin 2 ELISA Standard Curve. Recombinant Human Neuronal Pentraxin 2 protein was serially diluted 2-fold and captured by Mouse Anti-Human Neuronal Pentraxin 2 Monoclonal Antibody (Catalog # MAB78161) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Neuronal Pentraxin 2 Monoclonal Antibody (Catalog # MAB78162) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Neuronal Pentraxin 2

Neuronal Pentraxin (NPTX2), also called NP2 or NARP (neuronal activity-regulated pentraxin) is a 46-60 kDa secreted glycoprotein within the Pentraxin family (1-4). Neuronal pentraxins include NPTX1 and NPTX2, both secreted proteins, and NPTXR (Neuronal Pentraxin Receptor), which is found in type II transmembrane or cleaved, soluble forms (1-5). Circulating NPTX2 forms disulfide-linked 250 kDa homopentamers, while at excitatory synapses it can form heteropentamers and larger clusters with NPTX1 and NPTXR (1-5). The clusters promote synaptogenesis by recruiting subunits of AMPA-type glutamate receptors (AMPAR) (4-6). Of the three neuronal pentraxins, only NPTX2 shows immediate-early expression induced by synaptic activity (5, 6). Human NPTX2 is synthesized as a 431 amino acid (aa) protein with a 15 aa signal sequence and a 416 aa secreted mature protein that contains a calcium binding Pentraxin domain. Mature human NPTX2 shares 96% aa sequence identity with mouse, rat, bovine, and canine NPTX2. Unlike other neuronal pentraxins, NPTX2 expression is not limited to neurons and NPTX2 protein is detected in the plasma (1, 7). It is prominent in the hippocampus, cerebral cortex, cerebellum, hypothalamus, posterior pituitary, and retina (2-8). It is found at excitatory synapses in parvalbumin-expressing interneurons and vasopressin- and orexin-expressing hypothalamic neurons (5-7). Its expression is increased in the substantia nigra in Parkinson’s disease, and it is present in Lewy bodies (9). NPTX2 shows calcium-dependent adhesion (3, 6). It promotes axon outgrowth in cortical explants, and formation, maturation and plasticity of synapses in vivo (3-6, 8).

References