Human Myeloperoxidase/MPO Antibody Summary
Ala49-Ser745
Accession # P05164
Applications
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody.
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Myeloperoxidase DuoSet ELISA Kit (Catalog # DY3174) for convenient development of a sandwich ELISA or the Human Myeloperoxidase Quantikine ELISA Kit (Catalog # DMYE00B) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/mab31741_human-myeloperoxidase-mab-clone-358621-elisa-1532021125857.jpg)
Human Myeloperoxidase/MPO ELISA Standard Curve. Recombinant Human Myeloperoxidase/MPO protein was serially diluted 2-fold and captured by Rat Anti-Human Myeloperoxidase/MPO Monoclonal Antibody (Catalog # MAB31741) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myeloperoxidase/MPO
Myeloperoxidase (MPO) is a hemeprotein that belongs to the XPO subfamily of the heme peroxidase superfamily. MPO is synthesized as a preproprotein that undergoes proteolytic processing to generate a disulfide-linked heterodimer of the N-terminal beta -subunit (12 kDa) and C-terminal alpha subunit (60 kDa). Active MPO is a tetramer of two beta -subunits and two alpha -subunits that are also disulfide-linked through the two alpha -subunits. MPO is stored in granules and is an abundant protein in neutrophils and monocytes. MPO is released upon activation to catalyze the formation of powerful oxidants such as hypochlorous acid, which kills microbes. Unprocessed pro-MPO can also be released. Human and mouse MPO share 87% amino acid sequence identity.
Product Datasheets
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