Human MS4A3 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6247F
Detection of MS4A3 in Human Whole Blood Monocytes by Flow Cytometry.
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Human MS4A3 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MS4A3 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 489433
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human MS4A3
Accession # NP_006129
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MS4A3 antibody in Human Whole Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of MS4A3 in Human Whole Blood Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human MS4A3 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB6247F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MS4A3

MS4A3, also known as HTm4, is an membrane protein that is a member of the MS4A family of four-transmembrane proteins that also includes CD20 and Fc epsilon RI beta.  MS4A3 is a cell cycle regulator expressed in the perinuclear area of human basophils, eosinophils, early embryonic neural progenitors, splenic red pulp macrophages, monocytes and bile duct plus pancreatic duct epithelium. The C-terminal region of MS4A3 interacts with cdc-associated phosphatase (KAP), enhancing its activity on cyclin-dependent kinase 2 (cdk2) and facilitating G(0)/G(1) arrest. The combined non-membrane segments of the 214 amino acid (aa), 22-25 kDa human MS4A3 show 53% and 52% aa identity with corresponding regions of mouse and rat MS4A3, respectively.

Long Name
Membrane-spanning 4-domains, Subfamily A, Member 3 [Hematopoietic Cell-specific]
Entrez Gene IDs
932 (Human); 170813 (Mouse); 293753 (Rat)
Alternate Names
CD20 antigen homolog; CD20 antigen-like protein; CD20L; CD20LIgE receptor beta subunit; hematopoietic cell 4 transmembrane protein; Hematopoietic-specific transmembrane protein 4; HTM4; HTM4IgE receptor beta chain; membrane-spanning 4-domains subfamily A member 3; membrane-spanning 4-domains, subfamily A, member 3 (hematopoieticcell-specific); MS4A3

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