Human/Mouse VAMP-1/VAMP-2 Antibody

Catalog #: MAB5958 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5958
MAB5958-SP

Detection of Human/Mouse VAMP‑1/VAMP‑2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse VAMP-1/VAMP-2 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse VAMP-1/VAMP-2 in Western blots. This antibody does not react with recombinant VAMP family members 5, 7, and 8.

Source

Monoclonal Mouse IgG₁ Clone # 540524

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human VAMP-1
Met1-Lys96
Accession # P23763

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (globus pallidus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse VAMP‑1/VAMP‑2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse VAMP-1/VAMP-2 Monoclonal Antibody (Catalog # MAB5958) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-1/VAMP-2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VAMP-1/VAMP-2

VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1‑96) and a 22 aa transmembrane domain (aa 97‑118). There is one coiled-coil region between aa 33‑93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1‑96, human VAMP-1 is 98% aa identical to mouse VAMP-1.

Long Name

Vesicle-Associated Membrane Protein 1/2

Entrez Gene IDs

6843 (Human); 22317 (Mouse); 25624 (Rat)

Alternate Names

SPAX1; SYB1; VAMP1; VAMP-1; VAMP-1/VAMP-2; vesicle associated membrane protein 1