Human/Mouse TRAP220 Antibody

Catalog # Availability Size / Price Qty
AF5520
AF5520-SP
Detection of Human TRAP220 by Western Blot.
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Product Details
Citations (1)
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Supplemental Products
Reviews (1)

Human/Mouse TRAP220 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human TRAP220 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TRAP220
Phe878-Phe1031
Accession # Q15648
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TRAP220 antibody by Western Blot. View Larger

Detection of Human TRAP220 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse TRAP220 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5520) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRAP220 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAP220

TRAP220 (Thyroid hormone receptor associated protein 220 kDa) is a widely expressed member of the Mediator complex subunit 1 family of proteins. It constitutes one of some 25 subunits that make up the Mediator complex. Here, it serves as a scaffold protein that links nuclear hormone receptors to RNA polymerase II. Human TRAP220 is 1581 amino acids (aa) in length. It contains a Mediator complex interaction region (aa 108‑390) and two nuclear hormone receptor binding boxes (aa 600‑670). Phosphorylation of Thr1032 and Thr1457 materially increases TRAP220 activity. There are at least two splice variants. One shows a 22 aa substitution for aa 756‑1581 while another shows a nine aa substitution for aa 548‑1581. Over aa 878‑1031, human TRAP220 shares 92% aa identity with mouse TRAP220.

Long Name
Thyroid Hormone Receptor-associated Protein Complex Component 220 kDa
Entrez Gene IDs
5469 (Human); 19014 (Mouse); 497991 (Rat)
Alternate Names
Activator-recruited cofactor 205 kDa component; ARC205; CRSP1TRIP2; CRSP200p53 regulatory protein RB18A; DRIP230; DRIP230PPAR-binding protein; MBD4; MED1; mediator complex subunit 1DRIP205; mediator of RNA polymerase II transcription subunit 1; PBPPPARGBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPARBP; PPARBPMGC71488; PPARG binding protein; PPARGBP; RB18ATR-interacting protein 2; Thyroid hormone receptor-associated protein complex 220 kDa component; thyroid hormone receptor-associated protein complex component TRAP220; thyroid receptor interacting protein 2; Thyroid receptor-interacting protein 2; TRAP220; TRAP220TRIP-2; TRIP2; vitamin D receptor-interacting protein 230 kD; Vitamin D receptor-interacting protein complex component DRIP205

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Citation for Human/Mouse TRAP220 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. MiR137 is an androgen regulated repressor of an extended network of transcriptional coregulators
    Authors: Emeli M. Nilsson, Kristian B. Laursen, Jonathan Whitchurch, Andrew McWilliam, Niels Ødum, Jenny L. Persson et al.
    Oncotarget

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Reviews for Human/Mouse TRAP220 Antibody

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Human/Mouse TRAP220 Antibody
By Anonymous on 04/06/2022
Application: WB Sample Tested: Liver cells Species: Human