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Human/Mouse TOP2B Antibody

Catalog #: MAB6348
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6348
MAB6348-SP
Detection of Human and Mouse TOP2B by Western Blot.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse TOP2B Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human TOP2B in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75% cross-reactivity with recombinant mouse TOP2B and no cross-reactivity with recombinant human TOP2A is observed.
Source
Monoclonal Mouse IgG2b Clone # 681417
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse TOP2B antibody by Western Blot. View Larger

Detection of Human and Mouse TOP2B by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, K562 human chronic myelogenous leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse TOP2B Monoclonal Antibody (Catalog # MAB6348) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TOP2B at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TOP2B antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

TOP2B in HeLa Human Cell Line. TOP2B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse TOP2B Monoclonal Antibody (Catalog # MAB6348) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: TOP2B

TOP2B (DNA Topoisomerase II beta) is a 180-185 kDa member of the type IIA subfamily, topoisomerase family of molecules. It is ubiquitously expressed, and represents the larger of two known topoisomerases (the 2A/a-form being 170 kDa in size). TOP2A is essential for cell division, while TOP2B is active postmitotically. In an ATP-dependent manner, homodimeric TOP2B relives torsional stress created in DNA during transcription or as a consequence of replication. TOP2B first induces cleavage of double-stranded DNA, creating a space that allows for the physical repositioning of chromatin and a reduction in tension. This is followed by closure and ligation of the cleaved ends to recreate the original DNA structure. Human TOP2B is 1626 amino acids (aa) in length. It contains an ATPase domain (aa 101-201), an NES (aa 1034-1044), more that 30 Ser/Thr phosphorylation sites and a C-terminal DTHCT region (aa 1508-1611). There is one splice variant that shows a deletion of aa 24-28. Over aa 1187-1621, human TOP2B shares 91% aa identity with mouse TOP2B.               

Long Name
DNA Topoisomerase II beta
Entrez Gene IDs
7155 (Human); 21974 (Mouse)
Alternate Names
antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; EC 5.99.1.3; TOP2B; top2beta; TOPIIB; topo II beta; topoisomerase (DNA) II beta (180kD); topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen

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Citations for Human/Mouse TOP2B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy
    Authors: T Magdy, Z Jiang, M Jouni, H Fonoudi, D Lyra-Leite, G Jung, M Romero-Tej, HH Kuo, KA Fetterman, M Gharib, BT Burmeister, M Zhao, Y Sapkota, CJ Ross, BC Carleton, D Bernstein, PW Burridge
    Cell Stem Cell, 2021-09-15;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses
    Authors: Julien Oury, Yun Liu, Ana Töpf, Slobodanka Todorovic, Esthelle Hoedt, Veeramani Preethish-Kumar et al.
    Journal of Cell Biology
  3. Proteasomal inhibition potentiates drugs targeting DNA topoisomerase II
    Authors: Ka C. Lee, Rebecca L. Bramley, Ian G. Cowell, Graham H. Jackson, Caroline A. Austin
    Biochemical Pharmacology

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