Human/Mouse TGF-beta RI/ALK-5 Antibody Summary
Ala21-Glu121
Accession # BAA05023
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse TGF-beta RI/ALK-5 by Western Blot TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse TGF-beta RI/ALK-5 by Western Blot TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of Mouse TGF-beta RI/ALK-5 by Flow Cytometry TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RI/ALK-5
Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor‑like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.
- Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
- Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.
Product Datasheets
Citations for Human/Mouse TGF-beta RI/ALK-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Cholesterol Pathway Inhibition Induces TGF-beta Signaling to Promote Basal Differentiation in Pancreatic Cancer
Authors: Gabitova-Cornell L, Surumbayeva A, Peri S et al.
Cancer Cell
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Cationic proteins from eosinophils bind bone morphogenetic protein receptors promoting vascular calcification and atherogenesis
Authors: Meng Z, Zhang S, Li W et al.
European heart journal
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Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis
Authors: Wu X, Zhang D, Qiao X et al.
The European respiratory journal
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TGF-?1 induces PD-1 expression in macrophages through SMAD3-STAT3 cooperative signaling in chronic inflammation
Authors: Lei, Z;Tang, R;Wu, Y;Mao, C;Xue, W;Shen, J;Yu, J;Wang, X;Qi, X;Wei, C;Xu, L;Zhu, J;Li, Y;Zhang, X;Ye, C;Chen, X;Yang, X;Zhou, S;Su, C;
JCI insight
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis
Authors: Wu X, Zhang D, Qiao X et al.
The European respiratory journal
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Role of Nox4 in mitigating inflammation and fibrosis in dextran sulfate sodium-induced colitis
Authors: Lee, Y;Kim, SH;Jeong, H;Kim, KH;Jeon, D;Cho, Y;Lee, D;Nam, KT;
Cellular and molecular gastroenterology and hepatology
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Prevention of bleomycin-induced pulmonary fibrosis by a RANKL peptide in mice
Authors: N Ju, H Hayashi, M Shimamura, S Baba, S Yoshida, R Morishita, H Rakugi, H Nakagami
Scientific Reports, 2022-07-21;12(1):12474.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Fibulin-6 regulates pro-fibrotic TGF-beta responses in neonatal mouse ventricular cardiac fibroblasts.
Authors: Chowdhury A, Hasselbach L, Echtermeyer F, Jyotsana N, Theilmeier G, Herzog C
Sci Rep, 2017-02-17;7(0):42725.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis.
Authors: Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J
Nat Commun, 2016-03-18;7(0):11012.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Beclin 1 regulates neuronal transforming growth factor-beta signaling by mediating recycling of the type I receptor ALK5.
Authors: O'Brien C, Bonanno L, Zhang H, Wyss-Coray T
Mol Neurodegener, 2015-12-21;10(1):69.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-Fr -
Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
Proc Natl Acad Sci U S A, 2015-02-17;112(9):E957-65.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Integrin alpha3beta1 potentiates TGFbeta-mediated induction of MMP-9 in immortalized keratinocytes.
Authors: Lamar JM, Iyer V, DiPersio CM
J. Invest. Dermatol., 2007-08-30;128(3):575-86.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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