Home / SSEA-4 / Human/Mouse SSEA-4 Antibody

Human/Mouse SSEA-4 Antibody

Catalog #: MAB1435
66 Citations 9 Reviews Datasheet / COA / SDS
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MAB1435
MAB1435-SP
Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (66)
FAQs
Supplemental Products
Reviews (9)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in MES with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-4 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry SSEA‑4 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

SSEA‑4 in BG01V Human Stem Cells. SSEA-4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry Detection of Canine SSEA-4 by Flow Cytometry View Larger

Detection of Canine SSEA-4 by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine SSEA-4 by Flow Cytometry View Larger

Detection of Canine SSEA-4 by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine Human/Mouse SSEA-4 Antibody by Flow Cytometry View Larger

Detection of Canine Human/Mouse SSEA-4 Antibody by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine Human/Mouse SSEA-4 Antibody by Flow Cytometry View Larger

Detection of Canine Human/Mouse SSEA-4 Antibody by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References
  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4; SSEA-4; SSEA-4;stage-specific embryonic antigen 4

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Citations for Human/Mouse SSEA-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

66 Citations: Showing 1 - 10
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  1. Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused Three-Dimensional Multicompartment Bioreactor
    Authors: Nora Freyer, Fanny Knöspel, Nadja Strahl, Leila Amini, Petra Schrade, Sebastian Bachmann et al.
    BioResearch Open Access
  2. Reduced PLP1 expression in induced pluripotent stem cells derived from a Pelizaeus–Merzbacher disease patient with a partial PLP1 duplication
    Authors: Keiko Shimojima, Takahito Inoue, Yuki Imai, Yasuhiro Arai, Yuta Komoike, Midori Sugawara et al.
    Journal of Human Genetics
  3. Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant
    Authors: Nambiar TS, Billon P, Diedenhofen G et al.
    Nat Commun
  4. iPSC-derived beta cells model diabetes due to glucokinase deficiency.
    Authors: Hua H, Shang L, Martinez H et al.
    J Clin Invest
  5. Evaluation of the Age- and Sex-Related Changes of the Osteogenic Differentiation Potentials of Healthy Bone Marrow-Derived Mesenchymal Stem Cells
    Authors: Hyun-Jin Lee, Hyuna Lee, Chae-Bin Na, In-Seok Song, Jae-Jun Ryu, Jun-Beom Park
    Medicina (Kaunas)
  6. Generation and characterization of integration-free induced pluripotent stem cells from patients with autoimmune disease
    Authors: Mi-Young Son, Mi-Ok Lee, Hyejin Jeon, Binna Seol, Jung Hwa Kim, Jae-Suk Chang et al.
    Experimental & Molecular Medicine
  7. Verification and rectification of cell type-specific splicing of a Seckel syndrome-associated ATR mutation using iPS cell model
    Authors: Jose Ichisima, Naoya M. Suzuki, Bumpei Samata, Tomonari Awaya, Jun Takahashi, Masatoshi Hagiwara et al.
    Journal of Human Genetics
  8. Induced Pluripotent Stem Cells with Six Reprogramming Factors from Prairie Vole, Which is an Animal Model for Social Behaviors
    Authors: Masafumi Katayama, Takashi Hirayama, Kengo Horie, Tohru Kiyono, Kenichiro Donai, Satoru Takeda et al.
    Cell Transplantation
  9. Transcriptomic analysis of feeder-free culture system for maintaining naïve-state pluripotency in human pluripotent stem cells
    Authors: Wataru Isono, Tomoyuki Kawasaki, Justin K. Ichida, Kazunori Nagasaka, Osamu Hiraike, Akihiro Umezawa et al.
    Stem Cell Investigation
  10. Alternative splicing is a developmental switch for hTERT expression
    Authors: Penev A, Bazley A, Shen M et al.
    Mol Cell
  11. Renal primordia activate kidney regenerative events in a rat model of progressive renal disease.
    Authors: Imberti B, Corna D, Rizzo P, Xinaris C, Abbate M, Longaretti L, Cassis P, Benedetti V, Benigni A, Zoja C, Remuzzi G, Morigi M
    PLoS ONE, 2015-03-26;10(3):e0120235.
  12. Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States
    Authors: AJ Collier, SP Panula, JP Schell, P Chovanec, A Plaza Reye, S Petropoulo, AE Corcoran, R Walker, I Douagi, F Lanner, PJ Rugg-Gunn
    Cell Stem Cell, 2017-03-23;0(0):.
  13. Cell-type dependent regulation of pluripotency and chromatin remodeling genes by hydralazine
    Authors: Alain Aguirre-Vázquez, Fabiola Castorena-Torres, Beatriz Silva-Ramírez, Katia Peñuelas-Urquides, María Elena Camacho-Moll, Luis A. Salazar-Olivo et al.
    Stem Cell Research & Therapy
  14. A secreted proteomic footprint for stem cell pluripotency
    Authors: Lewis, PA;Silajdži?, E;Smith, H;Bates, N;Smith, CA;Mancini, FE;Knight, D;Denning, C;Brison, DR;Kimber, SJ;
    PloS one
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  15. High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
    Authors: Hung, TH;Huang, Y;Yeh, CT;Yeh, CN;Yu, J;Lin, CC;Chiou, SP;Chiang, PY;Hung, JT;Yu, AL;
    Biomedical journal
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. Derivation of Limbal Stem Cells from Human Adult Mesenchymal Stem Cells for the Treatment of Limbal Stem Cell Deficiency
    Authors: M Cadenas-Ma, F Arnalich-M, MP Miguel
    International Journal of Molecular Sciences, 2023-01-25;24(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
    Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
    Oncogene, 2022-07-11;63(0):102865.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  18. Generation of iPSC line (FAMRCi009-A) from patient with familial progressive cardiac conduction disorder carrying genetic variant FLNC p.Val2264Met
    Authors: N Rodina, A Khudiakov, K Perepelina, A Muravyev, A Boytsov, A Zlotina, P Sokolnikov, A Kostareva
    Stem Cell Research, 2021-12-27;59(0):102640.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  19. Generation of iPSC line FAMRCi010-A from patient with restrictive cardiomyopathy carrying genetic variant FLNC p.Gly2011Arg
    Authors: K Perepelina, A Khudiakov, N Rodina, A Boytsov, T Vavilova, A Zlotina, P Sokolnikov, A Kostareva
    Stem Cell Research, 2021-12-21;59(0):102639.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  20. Comparative evaluation of isogenic mesodermal and ectomesodermal chondrocytes from human iPSCs for cartilage regeneration
    Authors: MS Lee, MJ Stebbins, H Jiao, HC Huang, EM Leiferman, BE Walczak, SP Palecek, EV Shusta, WJ Li
    Science Advances, 2021-05-19;7(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Grafted human induced pluripotent stem cells improve the outcome of spinal cord injury: modulation of the lesion microenvironment
    Authors: T Bellák, Z Fekécs, D Török, Z Táncos, C Nemes, Z Tézsla, L Gál, S Polgári, J Kobolák, A Dinnyés, A Nógrádi, K Pajer
    Scientific Reports, 2020-12-29;10(1):22414.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human na�ve-state pluripotency in non-hypoxic conditions
    Authors: W Isono, T Kawasaki, JK Ichida, T Ayabe, O Hiraike, A Umezawa, H Akutsu
    Regenerative Therapy, 2020-09-02;15(0):161-168.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Generation of two iPSC lines (FAMRCi007-A and FAMRCi007-B) from patient with Emery-Dreifuss muscular dystrophy and heart rhythm abnormalities carrying genetic variant LMNA p.Arg249Gln
    Authors: K Perepelina, A Kostina, P Klauzen, A Khudiakov, M Rabino, S Crasto, A Zlotina, Y Fomicheva, A Sergushich, M Oganesian, A Dmitriev, A Kostareva, E Di Pasqual, A Malashiche
    Stem Cell Res, 2020-06-29;47(0):101895.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  24. Generation of two induced pluripotent stem cell lines (FAMRCi005-A and FAMRCi005-B) from patient carrying genetic variant LMNA p.Asp357Val
    Authors: P Klauzen, K Perepelina, A Khudiakov, A Zlotina, Y Fomicheva, T Pervunina, T Vershinina, A Kostareva, A Malashiche
    Stem Cell Res, 2020-02-05;43(0):101719.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  25. Generation of two iPSC lines (FAMRCi004-A and FAMRCi004-B) from patient with familial progressive cardiac conduction disorder carrying genetic variant DSP p.His1684Arg
    Authors: A Khudiakov, K Perepelina, P Klauzen, A Zlotina, K Gusev, E Kaznacheye, A Malashiche, A Kostareva
    Stem Cell Res, 2020-02-04;43(0):101720.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IF/ICC
  26. Generation of two iPSC lines (FAMRCi006-A and FAMRCi006-B) from patient with dilated cardiomyopathy and Emery-Dreifuss muscular dystrophy associated with genetic variant LMNAp.Arg527Pro
    Authors: K Perepelina, P Klauzen, A Khudiakov, A Zlotina, Y Fomicheva, D Rudenko, M Gordeev, A Sergushich, A Malashiche, A Kostareva
    Stem Cell Res, 2020-01-31;43(0):101714.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Activation of PDGF pathway links LMNA mutation to dilated cardiomyopathy
    Authors: J Lee, V Termglinch, S Diecke, I Itzhaki, CK Lam, P Garg, E Lau, M Greenhaw, T Seeger, H Wu, JZ Zhang, X Chen, IP Gil, M Ameen, K Sallam, JW Rhee, JM Churko, R Chaudhary, T Chour, PJ Wang, MP Snyder, HY Chang, I Karakikes, JC Wu
    Nature, 2019-07-17;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  28. Ciliary gene RPGRIP1L is required for hypothalamic arcuate neuron development
    Authors: L Wang, AJ De Solis, Y Goffer, KE Birkenbach, SE Engle, R Tanis, JM Levenson, X Li, R Rausch, M Purohit, JY Lee, J Tan, MC De Rosa, CA Doege, HL Aaron, GJ Martins, JC Brüning, D Egli, R Costa, N Berbari, RL Leibel, G Stratigopo
    JCI Insight, 2019-02-07;4(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  29. Effects of the Extracts from Fruit and Stem of Camellia japonica on Induced Pluripotency and Wound Healing
    Authors: H Jeon, JY Kim, JK Choi, E Han, CL Song, J Lee, YS Cho
    J Clin Med, 2018-11-20;7(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  30. Small non-coding RNA landscape of extracellular vesicles from human stem cells
    Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
    Sci Rep, 2018-10-19;8(1):15503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  31. DMSO increases efficiency of genome editing at two non-coding loci
    Authors: G Stratigopo, MC De Rosa, CA LeDuc, RL Leibel, CA Doege
    PLoS ONE, 2018-06-04;13(6):e0198637.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  32. High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma
    Authors: HH Kuo, RJ Lin, JT Hung, CB Hsieh, TH Hung, FY Lo, MY Ho, CT Yeh, YL Huang, J Yu, AL Yu
    Sci Rep, 2017-09-07;7(1):10750.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  33. Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics
    Authors: NG Kooreman, PE de Almeida, JP Stack, RV Nelakanti, S Diecke, NY Shao, RJ Swijnenbur, V Sanchez-Fr, E Matsa, C Liu, AJ Connolly, JF Hamming, PHA Quax, MA Brehm, DL Greiner, LD Shultz, JC Wu
    Cell Rep, 2017-08-22;20(8):1978-1990.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene
    Authors: A Khudiakov, D Kostina, A Zlotina, T Nikulina, A Sergushich, A Gudkova, A Tomilin, A Malashiche, A Kostareva
    Stem Cell Res, 2017-08-19;24(0):77-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Effect of microgravity on the mesenchymal stem cell characteristics of limbal fibroblasts
    Authors: SI Pao, KH Chien, HT Lin, MC Tai, JT Chen, CM Liang
    J Chin Med Assoc, 2017-05-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017-03-07;20(0):105-114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases
    Authors: I Karakikes, V Termglinch, DA Cepeda, J Lee, S Diecke, A Hendel, I Itzhaki, M Ameen, R Shrestha, H Wu, N Ma, NY Shao, T Seeger, NA Woo, KD Wilson, E Matsa, MH Porteus, V Sebastiano, JC Wu
    Circ. Res, 2017-02-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  38. Modeling Glanzmann thrombasthenia using patient specific iPSCs and restoring platelet aggregation function by CD41 overexpression
    Authors: L Hu, L Du, Y Zhao, W Li, Q Ouyang, D Zhou, G Lu, G Lin
    Stem Cell Res, 2017-02-11;20(0):14-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  39. The effects of glomerular and tubular renal progenitors and derived extracellular vesicles on recovery from acute kidney injury
    Authors: A Ranghino, S Bruno, B Bussolati, A Moggio, V Dimuccio, M Tapparo, L Biancone, P Gontero, B Frea, G Camussi
    Stem Cell Res Ther, 2017-02-07;8(1):24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  40. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
    Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
    PLoS ONE, 2016-04-04;11(4):e0152993.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  41. CXCR2 and its related ligands play a novel role in supporting the pluripotency and proliferation of human pluripotent stem cells.
    Authors: Jung J, Lee S, Kim J, Lee S, Sung H, An J, Park Y, Kim B
    Stem Cells Dev, 2014-12-23;24(8):948-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
    Authors: Luo, Lan, Kawakatsu, Miho, Guo, Chao-Wan, Urata, Yoshishi, Huang, Wen-Jing, Ali, Haytham, Doi, Hanako, Kitajima, Yuriko, Tanaka, Takayuki, Goto, Shinji, Ono, Yusuke, Xin, Hong-Bo, Hamano, Kimikazu, Li, Tao-Shen
    Sci Rep, 2014-01-21;4(0):3779.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  43. Characterization and molecular profiling of PSEN1 familial Alzheimer's disease iPSC-derived neural progenitors.
    Authors: Sproul A, Jacob S, Pre D, Kim S, Nestor M, Navarro-Sobrino M, Santa-Maria I, Zimmer M, Aubry S, Steele J, Kahler D, Dranovsky A, Arancio O, Crary J, Gandy S, Noggle S
    PLoS ONE, 2014-01-08;9(1):e84547.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. Telomerase-mediated telomere elongation from human blastocysts to embryonic stem cells.
    Authors: Zeng S, Liu L, Sun Y, Xie P, Hu L, Yuan D, Chen D, Ouyang Q, Lin G, Lu G
    J Cell Sci, 2013-12-11;127(0):752-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  45. Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
    Authors: Hayashi, Ryuhei, Ishikawa, Yuki, Ito, Miyuki, Kageyama, Tomofumi, Takashiba, Kuniko, Fujioka, Tsuyoshi, Tsujikawa, Motokazu, Miyoshi, Hiroyuki, Yamato, Masayuki, Nakamura, Yukio, Nishida, Kohji
    PLoS ONE, 2012-09-24;7(9):e45435.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  46. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012-08-31;8(0):150.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  47. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2011-11-03;132(3):563-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  48. Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells.
    Authors: Kim SY, Kim MJ, Jung H
    Stem Cells Dev., 2011-08-29;21(8):1272-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  49. Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
    Authors: Emdad L, D'Souza SL, Kothari HP, Qadeer ZA, Germano IM
    Stem Cells Dev., 2011-07-26;21(3):404-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  50. A bioinformatic assay for pluripotency in human cells.
    Authors: Muller FJ, Schuldt BM, Williams R, Mason D, Altun G, Papapetrou EP, Danner S, Goldmann JE, Herbst A, Schmidt NO, Aldenhoff JB, Laurent LC, Loring JF
    Nat. Methods, 2011-03-06;8(4):315-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  51. Generation of HIV-1 resistant and functional macrophages from hematopoietic stem cell-derived induced pluripotent stem cells.
    Authors: Kambal A, Mitchell G, Cary W, Gruenloh W, Jung Y, Kalomoiris S, Nacey C, McGee J, Lindsey M, Fury B, Bauer G, Nolta JA, Anderson JS
    Mol. Ther., 2010-11-30;19(3):584-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  52. Tumor initiating potential of side population cells in human gastric cancer.
    Authors: Fukuda K, Saikawa Y, Ohashi M, Kumagai K, Kitajima M, Okano H, Matsuzaki Y, Kitagawa Y
    Int. J. Oncol., 2009-05-01;34(5):1201-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  53. Vitronectin promotes oligodendrocyte differentiation during neurogenesis of human embryonic stem cells.
    Authors: Gil JE, Woo DH, Shim JH, Kim SE, You HJ, Park SH, Paek SH, Kim SK, Kim JH
    FEBS Lett., 2009-01-20;583(3):561-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  54. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008-05-01;215(2):329-36.
    Species: Equine
    Sample Types: Whole Cells
    Applications: ICC
  55. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008-04-03;26(8):1931-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  56. Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells.
    Authors: Hohenstein KA, Pyle AD, Chern JY, Lock LF, Donovan PJ
    Stem Cells, 2008-03-06;26(6):1436-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  57. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007-02-01;25(2):490-9.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  58. Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.
    Authors: Josephson R, Ording CJ, Liu Y, Shin S, Lakshmipathy U, Toumadje A, Love B, Chesnut JD, Andrews PW, Rao MS, Auerbach JM
    Stem Cells, 2007-02-01;25(2):437-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  59. Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
    Authors: Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
    FEBS Lett., 2006-10-02;580(25):5869-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  60. Robust pluripotent stem cell expansion and cardiomyocyte differentiation via geometric patterning
    Authors: Frank B. Myers, Jason S. Silver, Yan Zhuge, Ramin E. Beygui, Christopher K. Zarins, Luke P. Lee et al.
    Integrative Biology
  61. Effect of culture medium on propagation and phenotype of corneal stroma–derived stem cells
    Authors: Laura E. Sidney, Matthew J. Branch, Harminder S. Dua, Andrew Hopkinson
    Cytotherapy
  62. Comparing modes of delivery of a combination of ion channel inhibitors for limiting secondary degeneration following partial optic nerve transection
    Authors: LM Toomey, CA Bartlett, N Gavriel, T McGonigle, M Majimbi, G Gopalasing, J Rodger, M Fitzgerald
    Sci Rep, 2019-10-25;9(1):15297.
  63. Protein corona formation moderates the release kinetics of ion channel antagonists from transferrin-functionalized polymeric nanoparticles†
    Authors: Priya S. R. Naidu, Eleanor Denham, Carole A. Bartlett, Terry McGonigle, Nicolas L. Taylor, Marck Norret et al.
    RSC Advances
  64. Derivation of Sendai-Virus-Reprogrammed Human iPSCs-Neuronal Precursors: In Vitro and In Vivo Post-grafting Safety Characterization
    Authors: M Shigyo, Y Kobayashi, O Platoshyn, S Marsala, T Kato, N Takamura, K Yoshida, A Kishino, M Bravo-Hern, S Juhas, J Juhasova, H Studenovsk, V Proks, JD Ciacci, M Marsala
    Cell Transplantation, 2023-01-01;32(0):9636897231163.
  65. Generation of Human‐Induced Pluripotent Stem Cells From Anterior Cruciate Ligament
    Authors: Steven Woods, Nicola Bates, Sara L. Dunn, Ferdinand Serracino‐Inglott, Tim E. Hardingham, Susan J. Kimber
    Journal of Orthopaedic Research

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Reviews for Human/Mouse SSEA-4 Antibody

Average Rating: 4.2 (Based on 9 Reviews)

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Human/Mouse SSEA-4 Antibody
By Anonymous on 08/23/2021
Application: Flow Sample Tested: iPS2 human induced pluripotent stem cells Species: Human
Flow Cytometry Human/Mouse SSEA-4 Antibody MAB1435
Human/Mouse SSEA-4 Antibody
By Anonymous on 08/17/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: differentiated corneal epithelial cells Species: Mouse

1:200 dilution worked well.

Immunocytochemistry/Immunofluorescence Human/Mouse SSEA-4 Antibody MAB1435
Human/Mouse SSEA-4 Antibody
By Julieta O'Flaherty on 12/11/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

I used to check for SSEA-4 in my iPSCs and it worked great

Immunocytochemistry/Immunofluorescence Human/Mouse SSEA-4 Antibody MAB1435
Human/Mouse SSEA-4 Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human/Mouse SSEA-4 Antibody MAB1435
Human/Mouse SSEA-4 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human/Mouse SSEA-4 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Human/Mouse SSEA-4 Antibody
By Anonymous on 11/07/2018
Application: ELISA Sample Tested: Plasma,Serum Species: Human and Mouse
Human/Mouse SSEA-4 Antibody
By Anonymous on 03/12/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: fibrotic liver Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse SSEA-4 Antibody MAB1435
Human/Mouse SSEA-4 Antibody
By Simon Liu on 11/23/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human
Immunocytochemistry/Immunofluorescence Human/Mouse SSEA-4 Antibody MAB1435