Human/Mouse Sonic Hedgehog N‑Terminus Antibody

Catalog #: MAB464
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB464
MAB464-SP

Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot.

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Citations (4)

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Human/Mouse Sonic Hedgehog N‑Terminus Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects the N-terminal peptide of human (amino acids 24-197) and mouse (amino acids 25-198) Shh in direct ELISAs and Western blots. Shows 50-100% cross-reactivity with the N-terminal peptides of recombinant mouse (rm) Dhh (amino acids 23‑198) and rmIhh (amino acids 66‑240) and no cross-reactivity with the C‑terminal peptides from rmShh (amino acids 199‑437), rmDhh (amino acids 199‑396), or rmIhh (amino acids 241‑449).

Source

Monoclonal Rat IgG_2A Clone # 171001

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Sonic Hedgehog
Cys25-Gly198 (Lys122Arg)
Accession # Q62226

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human brain tissue and mouse brain tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Monoclonal Antibody (Catalog # MAB464) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sonic Hedgehog/Shh

Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).

Entrez Gene IDs

6469 (Human); 20423 (Mouse)

Alternate Names

HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

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Citations for Human/Mouse Sonic Hedgehog N‑Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Sonic Hedgehog and WNT signaling regulate a positive feedback loop between intestinal epithelial and stromal cells to promote epithelial regeneration
Authors: Orzechowska-Licari EJ, Bialkowska AB, Yang VW
Cellular and molecular gastroenterology and hepatology
Pancreatic tumor cell secreted CCN1/Cyr61 promotes endothelial cell migration and aberrant neovascularization.
Authors: Maity, Gargi, Mehta, Smita, Haque, Inamul, Dhar, Kakali, Sarkar, Sandipto, Banerjee, Sushanta, Banerjee, Snigdha
Sci Rep, 2014-05-16;4(0):4995.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Neuroprotective effect of sonic hedgehog up-regulated in Schwann cells following sciatic nerve injury.
Authors: Hashimoto M, Ishii K, Nakamura Y, Watabe K, Kohsaka S, Akazawa C
J. Neurochem., 2008-09-11;107(4):918-27.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Wntless, a conserved membrane protein dedicated to the secretion of Wnt proteins from signaling cells.
Authors: Banziger C, Soldini D, Schutt C, Zipperlen P, Hausmann G, Basler K
Cell, 2006-05-05;125(3):509-22.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot