Human/Mouse SHIP Antibody

Catalog #: AF10117 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF10117-100
AF10117-SP

Detection of Human and Mouse SHIP by Western Blot.

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Human/Mouse SHIP Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse SHIP in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse SHIP
Glu874-Thr941
Accession # Q9ES52

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

2 µg/1 mg cell lysate

Cell lysate of U-937 cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse SHIP by Western Blot. Western blot shows lysates of BaF3 mouse pro-B cell line and RL human non-Hodgkin's lymphoma B cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse SHIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10117) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SHIP at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunoprecipitation

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Detection of SHIP by Immunoprecipitation. PMA-treated U-937 lysates were prepared and immunoprecipitation was performed using 2.0 µg of Goat Anti-Human/Mouse SHIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10117) pre-coupled to Dynabeads protein G. Immunoprecipitated SHIP was detected with Mouse Anti-SHIP Antibody.The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SHIP

SH2-containing inositol phosphatase (SHIP), also known as INPP5D, is a negative regulator of signal transduction in hematopoietic cells. Targeted disruption of SHIP in mice leads to a myeloproliferative disorder. Several laboratories have demonstrated the presence of multiple forms of SHIP, including 145 kDa, 135 kDa, and
C‑terminal truncated forms at 125 kDa and 110 kDa in some cell types.

Long Name

SH2-containing Inositol Phosphatase

Entrez Gene IDs

3635 (Human); 16331 (Mouse); 54259 (Rat)

Alternate Names

145kD; EC 3.1.3; hp51CNMGC142142; Inositol polyphosphate-5-phosphatase of 145 kDa; inositol polyphosphate-5-phosphatase, 145kDa; INPP5D; MGC104855; SHIP; SHIPMGC142140