Human/Mouse/Rat Syntaxin 7 Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Syntaxin 7 by Western Blot. Western blot shows recombinant human Syntaxin 12, 16, 1A, 1B2, 5, 6, 7, 8, and 1B1 (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band for Syntaxin 7 was detected at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human, Mouse, and Rat Syntaxin 7 by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, BaF3 mouse pro-B cell line, L1.2 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band for Syntaxin 7 was detected at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Syntaxin 7 in HeLa Human Cell Line. Syntaxin 7 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Syntaxin 7 in Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells. Syntaxin 7 was detected in immersion fixed Daudi Human Burkitt's Lymphoma Cell Line (Positive) and MOLT‑4 Human Acute Lymphoblastic Leukemia Cell Line (Negative) Cells using Sheep Anti-Human/Mouse/Rat Syntaxin 7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5478) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Preparation and Storage
Background: Syntaxin 7
Syntaxin 7 (STX7) is a widely expressed protein embedded in endosomal and lysosomal membranes, and serves as a component of the SNARE complex. STX7 is involved in endocytic trafficking from early endosomes to late endosomes and lysosomes. This is in contrast to STX8, which is involved in clathrin-independent vesicular transport. Human STX7 is a type IV single-pass transmembrane protein (very short exoplasmic C-terminus) that is 261 amino acids (aa) in length. It contains a coiled-coil region (aa 47‑69), a t-SNARE domain (aa 165‑227) that is likely involved in protein-protein interactions, and a short, two amino acid, C-terminal luminal sequence. Over aa 21‑187, human STX7 shares 97% aa identity with mouse STX7.
Product Datasheets
Citations for Human/Mouse/Rat Syntaxin 7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Degradation of dendritic cargos requires Rab7-dependent transport to somatic lysosomes
Authors: Chan Choo Yap, Laura Digilio, Lloyd P. McMahon, A. Denise R. Garcia, Bettina Winckler
Journal of Cell Biology
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Munc13-4 functions as a Ca2+ sensor for homotypic secretory granule fusion to generate endosomal exocytic vacuoles.
Authors: Sang Su Woo, Declan J James, Thomas F J Martin
Molecular Biology of the Cell, 2017-01-18;0(0):1939-4586.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Munc13-4 interacts with syntaxin 7 and regulates late endosomal maturation, endosomal signaling, and TLR9-initiated cellular responses
Authors: Jing He, Jennifer L. Johnson, Jlenia Monfregola, Mahalakshmi Ramadass, Kersi Pestonjamasp, Gennaro Napolitano et al.
Molecular Biology of the Cell
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Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation
Authors: Ildar Gabaev, James C. Williamson, Thomas W.M. Crozier, Thomas F. Schulz, Paul J. Lehner
Cell Reports
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