Human/Mouse/Rat STAT5b Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB15841
MAB15841-SP
Detection of Human, Mouse, and Rat STAT5b by Western Blot.
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Product Details
Citations (1)
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Human/Mouse/Rat STAT5b Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat STAT5b in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1032E
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human STAT5b synthetic peptide aa 777-787
Accession # P51692
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunoprecipitation
2 µg/1 mg cell lysate
Cell lysate of HAP1 human near-haploid cell line
Immunocytochemistry
5-25 µg/mL
See below
Knockout Validated
A) STAT5b is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT5b knockout HeLa cell line. B) STAT5b is specifically detected in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat STAT5b antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat STAT5b by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, M1 mouse myeloid leukemia cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat STAT5b Monoclonal Antibody (Catalog # MAB15841) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for STAT5b at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry STAT5b antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

STAT5b in HepG2 Human Cell Line. STAT5b was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit Anti-Human/Mouse/Rat STAT5b Monoclonal Antibody (Catalog # MAB15841) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human and Mouse STAT5b antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse STAT5b by Simple WesternTM. Simple Western lane view shows lysates of M1 mouse myeloid leukemia cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5b at approximately 90 kDa (as indicated) using 1 μg/mL of Rabbit Anti-Human/Mouse/Rat STAT5b Monoclonal Antibody (Catalog # MAB15841). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated STAT5b Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line. View Larger

STAT5b Specificity is Shown by Immunocytochemistry in Knockout Cell Line. STAT5b was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with IFN-alpha 1 but is not detected in STAT5b knockout (KO) HeLa cell line using Rabbit Anti-Human/Mouse/Rat STAT5b Monoclonal Antibody (Catalog # MAB15841) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Knockout Validated Western Blot Shows Human STAT5b Antibody Specificity by Using Knockout Cell Lines. View Larger

Western Blot Shows Human STAT5b Specificity by Using Knockout Cell Lines. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, and STAT6 KO HeLa cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat STAT5b Monoclonal Antibody (Catalog # MAB15841) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT5b at approximately 85 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the STAT5b knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated View Larger

Western Blot Shows Human STAT5b Specificity Using Knockout Cell Line. Western blot shows lysates of HAP1 human near-haploid cell line and STAT5b knockout HAP1 cell line (KO). Nitrocellulose membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat STAT5b Monoclonal Antibody (Catalog # MAB15841) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for STAT5b at approximately 85 kDa (as indicated) in the parental HAP1 cell line, but is not detectable in knockout HAP1 cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Immunoprecipitation View Larger

Detection of Stat5b by Immunoprecipitation. Immunoprecipitation was performed on cell lysate of HAP1 human near-haploid cell line using 2.0 μg of Rabbit Anti-Human Stat5b Monoclonal Antibody (Catalog # MAB15841) pre-coupled to protein G or protein A beads. Immunoprecipitated Stat5b was detected with a Mouse Anti-Stat5b antibody. The Ponceau stained transfers of each blot are shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT5b

Signal Transduction and Activator of Transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL‑22, IL-6, IFN-alpha ). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation, and survival.

Long Name
Signal Transducer and Activator of Transcription 5b
Entrez Gene IDs
6777 (Human); 20851 (Mouse)
Alternate Names
signal transducer and activator of transcription 5B; STAT5; STAT5b; transcription factor STAT5B

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Citation for Human/Mouse/Rat STAT5b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Janus kinase inhibitors ruxolitinib and baricitinib impair glycoprotein-VI mediated platelet function
    Authors: Iván Parra-Izquierdo, Alexander R. Melrose, Jiaqing Pang, Hari Hara Sudhan Lakshmanan, Stéphanie E. Reitsma, Sai Hitesh Vavilapalli et al.
    Platelets

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