Human/Mouse/Rat SHP-1 Antibody

Catalog #: AF1878
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1878
AF1878-SP

Detection of Human/Mouse/Rat SHP‑1 by Western Blot.

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All SHP-1 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat SHP-1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat SHP-1. The antibody does not detect SHP-2 in Western blots.

Source

Polyclonal Goat IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat SHP‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat SHP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1878) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active SHP-1 aa 205-595 (Catalog # 1878-SH) and Recombinant Human SHP-2 (Catalog # 1894-SH) (2 ng/lane) were included. A specific band for SHP-1 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human SHP‑1 by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SHP-1 at approximately 65 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat SHP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1878) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: SHP-1

Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and "motheaten viable” phenotypes that are severely autoimmune and immunodeficient (1). The enzyme is highly expressed in leukocyte cell types (2). SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors (3). Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity (4). SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor (5). A phosphopeptide containing the EGFR (Y992) sequence (R&D Systems, Catalog # ES006) can be used to measure the activity of SHP-1 by detecting the release of phosphate (R&D Systems, Catalog # DY996).

References

Tsui, H.W. et al. (1993) Nature Genet. 4:124.
Matthews, R.J. et al. (1992) Mol. Cell. Biol. 12:2396.
Burshtyn, D.N. et al. (1997) J. Biol. Chem. 272:13066.
Pei, D. et al. (1994) Biochemistry 33:15483.
Tomic, S. et al. (1995) J. Biol. Chem. 270:21277.

Long Name

Src Homology Region 2 Domain Phosphatase 1

Entrez Gene IDs

5777 (Human); 15170 (Mouse); 116689 (Rat)

Alternate Names

HCP; HCPH; HPTP1C; PTP1C; PTP-1C; PTPN6; SHP1; SHP-1; SHP-1L; SH-PTP1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human/Mouse/Rat SHP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

S100A8 induces IL-10 and protects against acute lung injury.
Authors: Hiroshima Y, Hsu K, Tedla N, Chung Y, Chow S, Herbert C, Geczy C
J Immunol, 2014-02-14;192(6):2800-11.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot