Human/Mouse/Rat SHIP Antibody

Name: Human/Mouse/Rat SHIP Antibody
Brand: R&D Systems
SKU: MAB2317
Rating: 3.7 (3 reviews)

Catalog #: MAB2317 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2317
MAB2317-SP

Detection of Human SHIP by Western Blot.

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Human/Mouse/Rat SHIP Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat SHIP in Western blots.

Source

Monoclonal Rat IgG2a Clone # 257812

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SHIP by Western Blot. Western blot shows lysates of TF-1 human erythroleukemic cell line. Gels were loaded with 10 µg (lane 1), 20 µg (lane 2), and 40 µg (lane 3). PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for SHIP was detected at approximately 148 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry

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SHIP in Mouse Splenocytes. SHIP was detected in immersion fixed mouse splenocytes using Rat Anti-Human/Mouse/Rat SHIP Monoclonal Antibody (Catalog # MAB2317) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: SHIP

SH2-containing inositol phosphatase (SHIP), also known as INPP5D, is a negative regulator of signal transduction in hematopoietic cells. Targeted disruption of SHIP in mice leads to a myeloproliferative disorder. Several laboratories have demonstrated the presence of multiple forms of SHIP, including 145 kDa, 135 kDa, and
C‑terminal truncated forms at 125 kDa and 110 kDa in some cell types.

Long Name

SH2-containing Inositol Phosphatase

Entrez Gene IDs

3635 (Human); 16331 (Mouse); 54259 (Rat)

Alternate Names

145kD; EC 3.1.3; hp51CNMGC142142; Inositol polyphosphate-5-phosphatase of 145 kDa; inositol polyphosphate-5-phosphatase, 145kDa; INPP5D; MGC104855; SHIP; SHIPMGC142140