Human/Mouse/Rat RNF2 Antibody

Catalog #: AF6065 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6065
AF6065-SP

Detection of Human, Mouse, and Rat RNF2 by Western Blot.

1 Image

All RNF2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat RNF2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat RNF2 in Western blots.

Source

Polyclonal Goat IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human, Mouse, and Rat RNF2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Hepa 1-6 mouse hepatoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RNF2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RNF2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: RNF2

RNF2 (RING [really interesting gene] finger protein 2/1B; also BAP-1, HIP-2 interacting protein 3 and DinG) is a 37 kDa member of the ring finger domain family of proteins. It is expressed in embryonic tissue, and serves as an E3 ubiquitin ligase within a polycomb complex that acts on histone H2A. In effect, RNF2 contributes to gene repression by blocking RNA pol II advance along gene promoters. RNF2 forms homodimers, and interacts with over a dozen proteins. Human RNF2 is 336 amino acids (aa) in length contains one Zn‑finger domain (aa 51‑91) and two phosphorylation sites at Ser168 and Ser203. There is one potential isoform that shows a deletion of aa 84‑155. Over aa 147‑228, human RNF2 shares 99% aa identity with mouse RNF2.

Long Name

Ring Finger Protein 2

Entrez Gene IDs

6045 (Human); 19821 (Mouse); 304850 (Rat)

Alternate Names

BAP-1; BAP1RING finger protein 1B; DING; DINGRING finger protein BAP-1; EC 6.3.2; HIPI3; HIPI3RING1BE3 ubiquitin-protein ligase RING2; Huntingtin-interacting protein 2-interacting protein 3; Protein DinG; ring finger protein 2HIP2-interacting protein 3; RING1B; RING2; RING2EC 6.3.2.-; RNF2