Human/Mouse/Rat Rheb Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human/Mouse/Rat Rheb antibody by Western Blot. Detection of Human/Mouse/Rat Rheb antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/Rheb_MAB3426_Western_Blot_6104.jpg)
Detection of Human/Mouse/Rat Rheb by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, Ramos human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Rheb at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Rheb antibody in Ramos Human Cell Line by Immunocytochemistry (ICC). Rheb antibody in Ramos Human Cell Line by Immunocytochemistry (ICC).](https://resources.rndsystems.com/images/datasheets/antibody/Rheb_MAB3426_Immunocytochemistry__Immunofluorescence_22496.jpg)
Rheb in Ramos Human Cell Line. Rheb was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
![](https://resources.rndsystems.com/images/datasheets/mab3426_human-mouse-rat-rheb-mab-clone-344912-immunocytochemistry-31102022134415.jpg)
Detection of Rheb in NIH‑3T3 mouse embryonic fibroblast cell line (Positive) & PC‑12 rat adrenal pheochromocytoma cell line (Negative). Rheb was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line (Positive) & PC‑12 rat adrenal pheochromocytoma cell line (Negative) using Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Preparation and Storage
Background: Rheb
Ras homolog enriched in brain (Rheb) is a novel member of the Ras superfamily of GTP-binding proteins involved in cell growth and cell cycle progression. Expressed in a wide range of tissues, Rheb was initially identified from its induction in rat hippocampal granule cells by seizures and NMDA-dependent synaptic activity. A complex containing the tuberous sclerosis gene products TSC1 and TSC2 functions as a GTPase-activating protein (GAP) for Rheb.
Product Datasheets
Citations for Human/Mouse/Rat Rheb Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers
Authors: Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J;
Biosensors
Species: Mouse
Sample Types: Complex Sample Type
Applications: IHC -
A beta-Cyclodextrin-Based Nanoparticle with Very High Transfection Efficiency Unveils siRNA-Activated TLR3 Responses in Human Prostate Cancer Cells
Authors: C de la Torr, P Játiva, I Posadas, D Manzanares, JLJ Blanco, CO Mellet, JMG Fernández, V Ceña
Pharmaceutics, 2022-11-09;14(11):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inactivation of Rheb by PRAK-mediated phosphorylation is essential for energy-depletion-induced suppression of mTORC1.
Authors: Zheng M, Wang YH, Wu XN, Wu SQ, Lu BJ, Dong MQ, Zhang H, Sun P, Lin SC, Guan KL, Han J
Nat. Cell Biol., 2011-02-20;13(3):263-72.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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