Human/Mouse/Rat PKA C Pan Specific Antibody

Catalog #: MAB4175
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4175
MAB4175-SP

Detection of Human/Mouse/Rat PKA C by Western Blot.

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All PKA C (pan) Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PKA C Pan Specific Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human PKA C alpha, C beta, and C gamma as well as endogenous human, mouse, and rat PKA C isoforms at 39-42 kDa using Western blot.

Source

Monoclonal Mouse IgG_2A Clone # 485013

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PKA C gamma
Met1-Phe351
Accession # ABM84853

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PKA C by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat PKA C Pan Specific Monoclonal Antibody (Catalog # MAB4175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKA C (pan)

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Long Name

cAMP-dependent Protein Kinase Catalytic Subunit

Alternate Names

CAFD1; PKA C; PKACA; PPNAD4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat PKA C Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Neuromedin B regulates steroidogenesis, cell viability and apoptosis in rabbit Leydig cells
Authors: Z Ma, J Guo, Y Zhang, Y Zhang, M Zhang, R Zong, F Chen, J Zhang
Gen. Comp. Endocrinol., 2019-12-16;288(0):113371.
Species: Rabbit
Sample Types: Cell Lysates
Applications: Western Blot
Phosphodiesterase 3B (PDE3B) antagonizes the anti-angiogenic actions of PKA in human and murine endothelial cells
Authors: JL MacKeil, P Brzezinska, J Burke-Klei, AL Theilmann, CJB Nicol, ML Ormiston, DH Maurice
Cell. Signal., 2019-06-05;62(0):109342.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot