Human/Mouse/Rat Neurolysin Antibody

Catalog #: AF3814 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3814
AF3814-SP

Detection of Human, Mouse, and Rat Neurolysin by Western Blot.

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All Neurolysin Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Neurolysin Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Neurolysin Neurolysin in Western blots and human Neurolysin in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Meprin alpha, rhMeprin beta subunit, and rhTHOP1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Neurolysin
aa 38-704

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Neurolysin (Catalog # 3814-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat Neurolysin by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line, rat cortical neuron tissue, and human brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Neurolysin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3814) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurolysin at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurolysin

Neurolysin, also known as Oligopeptidase M (mitochondrial peptidase), soluble angiotensin II-binding protein and endopeptidase EC 3.4.24.16 (1, 2), is a homologue of Thimet Ologopeptidase (THOP1), a zinc peptidase of the M3 family that also includes mitochondrial intermediate peptidase. Neurolysin expresses in two forms with the difference of an N-terminal transit peptide that targets the localization to mitochondria (1). The shorter form exists in cytosol. The recombinant human Neurolysin is expressed without the transit peptide and is isolated from the cytosol. Like THOP1, Neurolysin is capable of cleaving a number of vasoactive peptides such as bradykinin and neurotensin (3). All known substrates of THOP1 and Neurolysin contain 17 or fewer amino acids.

References

Serizawa, A. et al. (1995) J. Biol. Chem. 270:2092.
Barett, A.J. and J.M. Chen (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 356, Elsevier Academic Press, San Diego.
Norman, M.U. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1978.

Entrez Gene IDs

57486 (Human); 75805 (Mouse); 117041 (Rat)

Alternate Names

AGTBP; AGTBPFLJ23002; angiotensin binding protein; Angiotensin-binding protein; DKFZp564F123; EC 3.4.24; EC 3.4.24.16; KIAA1226EP24.16; MEP; mitochondrial; MOP; neurolysin (metallopeptidase M3 family); Neurolysin; Neurotensin Endopeptidase; NLN; Oligopeptidase M