Human/Mouse/Rat GRP78/HSPA5 Antibody Summary
Met1-Leu654
Accession # P11021
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Rat GRP78/HSPA5 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRP78/HSPA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4846) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRP78/HSPA5 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Human GRP78/HSPA5 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GRP78/HSPA5 at approximately 74 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat GRP78/HSPA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4846) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRP78/HSPA5
GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145‑245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 1‑10), and multiple single aa substitituion. Over aa 1‑654, human GRP78 is more than 97% aa identical to mouse and rat GRP78.
Product Datasheets
Citations for Human/Mouse/Rat GRP78/HSPA5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Cooperative treatment effectiveness of ATR and HSP90 inhibition in Ewing's sarcoma cells
Authors: C Marx, MU Schaarschm, J Kirkpatric, L Marx-Blüme, M Halilovic, M Westermann, D Hoelzer, FB Meyer, Y Geng, K Buder, HM Schadwinke, K Siniuk, S Becker, R Thierbach, JF Beck, J Sonnemann, ZQ Wang
Cell & bioscience, 2021-03-20;11(1):57.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease
Authors: KA Piróg, EP Dennis, CL Hartley, RM Jackson, J Soul, JM Schwartz, JF Bateman, RP Boot-Handf, MD Briggs
PLoS Genet., 2019-07-01;15(7):e1008215.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response
Authors: Paloma Gómez-Fernández, Andoni Urtasun, Adrienne W. Paton, James C. Paton, Francisco Borrego, Devin Dersh et al.
Frontiers in Immunology
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The Role of Autophagy in the Degradation of Misfolded HLA-B27 Heavy Chains
Authors: F Navid, G Layh-Schmi, KA Sikora, A Cougnoux, RA Colbert
Arthritis & rheumatology (Hoboken, N.J.), 2018-03-25;0(0):.
Species: Rat
Sample Types: Cell Lysate, Whole Cells
Applications: Immunoprecipitation, Western Blot -
The Novel Mechanisms Concerning the Inhibitions of Palmitate-Induced Proinflammatory Factor Releases and Endogenous Cellular Stress with Astaxanthin on MIN6 ?-Cells
Authors: A Kitahara, K Takahashi, N Morita, T Murashima, H Onuma, Y Sumitani, T Tanaka, T Kondo, T Hosaka, H Ishida
Mar Drugs, 2017-06-20;15(6):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-kappaappa B signaling induced by tumor necrosis factor-alpha in human A549 lung epithelial cells.
Authors: Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
PLoS ONE, 2014-06-24;9(6):e100787.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
Authors: Oida T, Weiner HL
J. Immunol., 2010-08-18;185(6):3529-35.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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