Human/Mouse/Rat GRK5 Antibody

Catalog #: AF4539
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4539
AF4539-SP

Detection of Human/Mouse/Rat GRK5 by Western Blot.

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Product Details

Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat GRK5 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat GRK5 in Western blots.

Source

Polyclonal Goat IgG

Immunogen

E. coli-derived recombinant human GRK5
Pro463-Ser590
Accession # P34947

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Knockout Validated

GRK5 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in GRK5 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat GRK5 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRK5 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human GRK5 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human GRK5 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and GRK5 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GRK5 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: GRK5

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.

Long Name

G protein-coupled Receptor Kinase 5

Entrez Gene IDs

2869 (Human); 14773 (Mouse); 59075 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.16; FLJ39780; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5; GPRK5; GPRK5FP2025; GRK5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat GRK5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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GPCR kinases generate an APH1A phosphorylation barcode to regulate amyloid-beta generation
Authors: NK Todd, Y Huang, JY Lee, P Doruker, JM Krieger, R Salisbury, M MacDonald, I Bahar, A Thathiah
Oncogene, 2022-07-19;40(3):111110.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Dissecting the roles of GRK2 and GRK3 in ?-opioid receptor internalization and &beta-arrestin2 recruitment using CRISPR/Cas9-edited HEK293 cells
Authors: TC Møller, MF Pedersen, JR van Senten, SD Seiersen, JM Mathiesen, M Bouvier, H Bräuner-Os
Sci Rep, 2020-10-15;10(1):17395.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Distinct cellular and subcellular distributions of G protein-coupled receptor kinase and arrestin isoforms in the striatum.
Authors: Bychkov, Evgeny, Zurkovsky, Lilia, Garret, Mika B, Ahmed, Mohamed, Gurevich, Eugenia
PLoS ONE, 2012-11-06;7(11):e48912.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
GRK5 promotes F-actin bundling and targets bundles to membrane structures to control neuronal morphogenesis.
Authors: Chen Y, Wang F, Long H
J. Cell Biol., 2011-09-19;194(6):905-20.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC