Human/Mouse/Rat FLIP Antibody

Catalog #: MAB8430
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8430
MAB8430-SP

Detection of Human, Mouse, and Rat FLIP by Western Blot.

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All FLIP Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FLIP Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human FLIP in direct ELISAs and human, mouse, and rat FLIP in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 896537

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FLIP
Met1-Asn200
Accession # O15519

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat FLIP by Western Blot. Western blot shows lysates of PANC-1 human pancreatic carcinoma cell line, L-929 mouse fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat FLIP Monoclonal Antibody (Catalog # MAB8430) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FLIP at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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FLIP in Human Pancreatic Cancer Tissue. FLIP was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human/Mouse/Rat FLIP Monoclonal Antibody (Catalog # MAB8430) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cells in islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLIP

FLIP (Flice-Like Inhibitory Protein), also known as CFLAR (CASP8 and FADD-Like Apoptosis Regulator), I-FLICE, CASPER, and FLAME-1, is an apoptosis inhibitory protein with architecture similar to that of Caspases-8 and -10. Both of the major FLIP isoforms, the 55 kDa Long (L) and the 27 kDa Short (S), contain two death effector domains (DED). FLIP(L) has a C-terminal Caspase-like protease domain which lacks both a catalytic active site and residues that form a substrate-binding pocket. FLIP(S) and FLIP(L) interact with the adaptor molecule FADD, and potently inhibit apoptosis initiated by Fas Ligand/TNF signaling pathways. Over amino acids 1-200, human FLIP shares 75% sequence identity with mouse and rat FLIP.

Long Name

FLICE Inhibitory Proteins

Entrez Gene IDs

8837 (Human); 12633 (Mouse)

Alternate Names

CASHFADD-like antiapoptotic molecule 1; CASP8 and FADD-like apoptosis regulator; CASP8AP1; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; CASPER; CasperFLAME1; Cellular FLICE-like inhibitory protein; CFLAR; c-FLIPR; c-FLIPS; CLARPMACH-related inducer of toxicity; FADD-like apoptotic molecule; FLAME; FLAME-1; FLIP; I-FLICE; I-FLICEMRITc-FLIPFLIPc-FLIPL; Inhibitor of FLICE; usurpin beta; Usurpin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat FLIP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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LILRB3 supports acute myeloid leukemia development and regulates T-cell antitumor immune responses through the TRAF2-cFLIP-NF-?B signaling axis
Authors: Wu G, Xu Y, Schultz RD et al.
Nature Cancer
Induction of immunosuppressive functions and NF-?B by FLIP in monocytes
Authors: A Fiore, S Ugel, F De Sanctis, S Sandri, G Fracasso, R Trovato, S Sartoris, S Solito, S Mandruzzat, F Vascotto, KL Hippen, G Mondanelli, U Grohmann, G Piro, C Carbone, D Melisi, RT Lawlor, A Scarpa, A Lamolinara, M Iezzi, M Fassan, S Bicciato, BR Blazar, U Sahin, PJ Murray, V Bronte
Nat Commun, 2018-12-05;9(1):5193.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC