Human/Mouse/Rat Fibromodulin/FMOD Antibody Summary
Asp75-Ile376
Accession # Q06828
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human/Mouse/Rat Fibromodulin antibody by Western Blot. Detection of Human/Mouse/Rat Fibromodulin antibody by Western Blot.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Fibromodulin_AF5945_Western_Blot_5889.jpg)
Detection of Human/Mouse/Rat Fibromodulin by Western Blot. Western blot shows lysates of human cartilage, human ovarian cancer, and rat uterine tissue. PVDF membrane was probed with 0.3 µg/mL of Sheep Anti-Human/Mouse/Rat Fibromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5945) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibromodulin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibromodulin/FMOD
Fibromodulin (FMOD; also FM and collagen-binding 59 kDa protein) is a secreted, 60‑70 kDa class II member of the SLRP family of matrix proteins. It is expressed by a variety of cell types, including keratinocytes, fibroblasts and chrondrocytes, and is known to bind to type I and XII collagen, plus TGF-beta. Fibromodulin is posited to displace lumican binding to collagen, generating 3D collagen fibrils from simple lumican-induced collagen dimers. Mature human Fibromodulin is 358 amino acids (aa) in length (aa 19‑376). It contains 12 LRRs (Leu-rich repeats) (aa 98‑376), with the seventh and eleventh LRR principally responsible for collagen type I binding. Fibromodulin contains varying amounts of keratan sulfate, and undergoes significant tyrosine sulfation. There is one splice form that shows a deletion of aa 88‑101. Over aa 76‑376, human Fibromodulin shares 96% aa identity with mouse and rat Fibromodulin.
Product Datasheets
Citation for Human/Mouse/Rat Fibromodulin/FMOD Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment
Authors: Kolb, J;Tsata, V;John, N;Kim, K;Möckel, C;Rosso, G;Kurbel, V;Parmar, A;Sharma, G;Karandasheva, K;Abuhattum, S;Lyraki, O;Beck, T;Müller, P;Schlü beta ler, R;Frischknecht, R;Wehner, A;Krombholz, N;Steigenberger, B;Beis, D;Takeoka, A;Blümcke, I;Möllmert, S;Singh, K;Guck, J;Kobow, K;Wehner, D;
Nature communications
Species: Fish - Danio rerio (Zebrafish)
Sample Types:
Applications: Immunofluorescence
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