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Human/Mouse/Rat alpha -Actinin 1 Antibody

Catalog #: AF8279
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF8279
AF8279-SP
Detection of Human, Mouse, and Rat alpha -Actinin 1 by Western Blot.
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Product Details
Citations (6)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat alpha -Actinin 1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat alpha -Actinin 1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human alpha -Actinin 1
Asp272-Thr500
Accession # P12814
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat a-Actinin 1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat alpha -Actinin 1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat a-Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a-Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry a-Actinin 1 antibody in A172 Human Cell Line by Immunocytochemistry (ICC). View Larger

alpha -Actinin 1 in A172 Human Cell Line. a-Actinin 1 was detected in immersion fixed A172 human glioblastoma cell line using Goat Anti-Human/Mouse/Rat a-Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha Actinin 1

Alpha-Actinin 1 (ACTN1) is a member of the spectrin superfamily with a predicted molecular weight of approximately 100 kDa.  It is 892 amino acids in length and shares 99% aa identity with mouse and rat ACTN1.  Multiple splice forms exist, resulting in 4 distinct isoforms with different tissue expression patterns.  ACTN1 binds to F-actin as well as other cytoskeletal and signaling proteins and plays an important role in both cell motility and muscle contraction.

Long Name
Actinin alpha 1
Entrez Gene IDs
87 (Human); 109711 (Mouse); 81634 (Rat)
Alternate Names
actinin 1 smooth muscle; actinin, alpha 1; ACTN1; alpha Actinin 1; alphaActinin 1; alpha-Actinin 1; Alpha-actinin cytoskeletal isoform; alpha-actinin-1; BDPLT15; F-actin cross-linking protein; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1

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Citations for Human/Mouse/Rat alpha -Actinin 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Cardiomyocyte Contractility and Autophagy in a Premature Senescence Model of Cardiac Aging.
    Authors: Haseli S, Deubel S et al.
    Oxid Med Cell Longev
  2. CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle
    Authors: Colpan M, Iwanski J, Gregorio CC
    Communications biology
  3. Poly (ADP-ribose) polymerases 16 triggers pathological cardiac hypertrophy via activating IRE1 alpha –sXBP1–GATA4 pathway
    Authors: Haibi Su, Jie Xu, Zhenghua Su, Chenxi Xiao, Jinghuan Wang, Wen Zhong et al.
    Cellular and Molecular Life Sciences
  4. SARS-CoV-2 Infection of Human Neurons Is TMPRSS2 Independent, Requires Endosomal Cell Entry, and Can Be Blocked by Inhibitors of Host Phosphoinositol-5 Kinase
    Authors: Pinja Kettunen, Angelina Lesnikova, Noora Räsänen, Ravi Ojha, Leena Palmunen, Markku Laakso et al.
    Journal of Virology
  5. The miR-106b/NR2F2-AS1/PLEKHO2 Axis Regulates Migration and Invasion of Colorectal Cancer through the MAPK Pathway
    Authors: Shuzhen Liu, Guoyan An, Qing Cao, Tong Li, Xinyu Jia, Lei Lei
    International Journal of Molecular Sciences
  6. Disruption of cardiac thin filament assembly arising from a mutation in LMOD2: A novel mechanism of neonatal dilated cardiomyopathy
    Authors: RC Ahrens-Nic, CT Pappas, GP Farman, RM Mayfield, TM Larrinaga, L Medne, A Ritter, ID Krantz, C Murali, KY Lin, JH Berger, SW Yum, CK Carreon, CC Gregorio
    Sci Adv, 2019-09-04;5(9):eaax2066.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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