Human/Mouse Phospho-Tie-2 (Y992) Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Mouse Phospho-Tie-2 (Y992) antibody by Western Blot. Detection of Mouse Phospho-Tie-2 (Y992) antibody by Western Blot.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Tie-2_AF2720_Western_Blot_5212.jpg)
Detection of Mouse Phospho-Tie‑2 (Y992) by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line transfected with mouse Tie-2 and untreated (-) or treated (+) with 600 ng/mL Recombinant Human Angiopoietin-1 (Catalog # 923-AN) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-Tie-2 (Y992) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Tie-2 (Y992) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
![Phospho-Tie-2 (Y992) antibody in Human Kidney Tissue by Immunohistochemistry (IHC-P). Phospho-Tie-2 (Y992) antibody in Human Kidney Tissue by Immunohistochemistry (IHC-P).](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Tie2_AF2720_Immunohistochemistry_24140.jpg)
Phospho-Tie-2 (Y992) in Human Kidney Tissue. Tie-2 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Rabbit Anti-Human/Mouse Phospho-Tie-2 (Y992) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2720) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC003) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
![Detection of Tie‑2 antibody in HUVEC Human Cells antibody by Flow Cytometry. Detection of Tie‑2 antibody in HUVEC Human Cells antibody by Flow Cytometry.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/Tie-2_AF2720_Flow_Cytometry_8182.jpg)
Detection of Tie‑2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were unstimulated (light orange filled histogram) or treated with 100 µM pervanadate for 15 minutes (dark orange filled histogram), then stained with Human/Mouse Phospho- Tie-2 (Y992) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2720) or control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tie-2
Tie-2 (also known as TEK) is an angiogenic receptor tyrosine kinase required for the later stage of blood vessel maturation. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Y992 is located on the putative activation loop of Tie-2 and is a major autophosphorylation site.
Product Datasheets
Citations for Human/Mouse Phospho-Tie-2 (Y992) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
23
Citations: Showing 1 - 10
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An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone
Authors: E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim
Experimental & Molecular Medicine, 2023-02-24;55(2):470-484.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Shear stress control of vascular leaks and atheromas through Tie2 activation by VE-PTP sequestration
Authors: K Shirakura, P Baluk, AF Nottebaum, U Ipe, KG Peters, DM McDonald, D Vestweber
Embo Molecular Medicine, 2023-02-06;0(0):e16128.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Adjunctive therapy with the Tie2 agonist Vasculotide reduces pulmonary permeability in Streptococcus pneumoniae infected and mechanically ventilated mice
Authors: A Lask, B Gutbier, O Kershaw, G Nouailles, AD Gruber, HC Müller-Red, S Chackowicz, DA Hamilton, P Van Slyke, M Witzenrath
Scientific Reports, 2022-09-15;12(1):15531.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain
Authors: YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong
Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764.
Species: Mouse
Sample Types: Protein Lysates
Applications: Western Blot -
Oligodendrocyte precursor cell specification is regulated by bidirectional neural progenitor-endothelial cell crosstalk
Authors: I Paredes, JR Vieira, B Shah, CF Ramunno, J Dyckow, H Adler, M Richter, G Schermann, E Giannakour, L Schirmer, HG Augustin, C Ruiz de Al
Nature Neuroscience, 2021-01-28;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Sulfated glycans engage the Ang-Tie pathway to regulate vascular development
Authors: ME Griffin, AW Sorum, GM Miller, WA Goddard, LC Hsieh-Wils
Nat Chem Biol, 2020-10-05;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting the ER&beta/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC
Authors: J Gu, Y Zhang, Z Han, L Gao, J Cui, Y Sun, Y Niu, B You, CP Huang, C Chang, X Wang, S Yeh
Cell Death Dis, 2020-05-14;11(5):367.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Preclinical validation of a novel metastasis-inhibiting Tie1 function-blocking antibody
Authors: M Singhal, N Gengenbach, S La Porta, S Gehrs, J Shi, M Kamiyama, DM Bodenmille, A Fischl, B Schieb, E Besemfelde, S Chintharla, HG Augustin
EMBO Mol Med, 2020-04-17;0(0):e11164.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Pulmonary pericytes regulate lung morphogenesis
Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
Nat Commun, 2018-06-22;9(1):2448.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Tie2 protects the vasculature against thrombus formation in systemic inflammation
Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
J. Clin. Invest., 2018-03-05;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Transcriptome Profiling of Neovascularized Corneas Reveals miR-204 as a Multi-target Biotherapy Deliverable by rAAVs
Authors: Y Lu, PWL Tai, J Ai, DJ Gessler, Q Su, X Yao, Q Zheng, PD Zamore, X Xu, G Gao
Mol Ther Nucleic Acids, 2017-12-30;10(0):349-360.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cooperation between ZEB2 and Sp1 promotes cancer cell survival and angiogenesis during metastasis through induction of survivin and VEGF
Authors: D Ko, S Kim
Oncotarget, 2017-12-11;9(1):726-742.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
J. Clin. Invest., 2017-09-18;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability
Authors: AV Shah, GM Birdsey, C Peghaire, ME Pitulescu, NP Dufton, Y Yang, I Weinberg, L Osuna Alma, L Payne, JC Mason, H Gerhardt, RH Adams, AM Randi
Nat Commun, 2017-07-11;8(0):16002.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Regulation of Angiopoietin Signalling by Soluble Tie2 Ectodomain and Engineered Ligand Trap
Authors: DOA Alawo, TA Tahir, M Fischer, DG Bates, SR Amirova, NPJ Brindle
Sci Rep, 2017-06-16;7(1):3658.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy
Authors: T Shlamkovic, L Aharon, WA Barton, N Papo
Oncotarget, 2017-05-16;8(20):33571-33585.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization
Authors: VM Leppänen, P Saharinen, K Alitalo
Proc. Natl. Acad. Sci. U.S.A., 2017-04-10;0(0):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Insertion of scFv into the hinge domain of full-length IgG1 monoclonal antibody results in tetravalent bispecific molecule with robust properties
Authors: Binyam Bezabeh
MAbs, 2017-02-01;0(0):0.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Angiopoetin-2 Signals Do Not Mediate the Hypervascularization of Islets in Type 2 Diabetes
PLoS ONE, 2016-09-12;11(9):e0161834.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
Authors: Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger M, Wu D, Min W
Nat Med, 2016-08-22;22(9):1033-1042.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Tie1 attenuation reduces murine atherosclerosis in a dose-dependent and shear stress-specific manner.
Authors: Woo KV, Qu X, Babaev VR, Linton MF, Guzman RJ, Fazio S, Baldwin HS
J. Clin. Invest., 2011-03-07;121(4):1624-35.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway.
Authors: Bai Y, Cui M, Meng Z, Shen L, He Q, Zhang X, Chen F, Xiao J
Biochem. Biophys. Res. Commun., 2008-11-24;378(2):296-301.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.
Authors: Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K
Nat. Cell Biol., 2008-04-20;10(5):527-37.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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