Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody

Name: Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody
Brand: R&D Systems
SKU: MAB892
Rating: 5 (1 reviews)

Catalog #: MAB892 1 Review Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB892
MAB892-SP

Detection of Human Phospho-RSK1 (S221)/RSK2 (S227) by Western Blot.

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Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse RSK1 when phosphorylated at S221, and human and mouse RSK2 when phosphorylated at S227.

Source

Monoclonal Rabbit IgG Clone # 1019B

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-RSK1 (S221)/RSK2 (S227) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Monoclonal Antibody (Catalog # MAB892) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phopho-RSK1 (S221)/RSK2 (S227) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Phospho-RSK1 (S221)/RSK2 (S227) by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, loaded at 0.1 mg/mL. A specific band was detected for Phospho-RSK1 (S221)/RSK2 (S227) at approximately 91 kDa (as indicated) using 5 μg/mL of Rabbit Anti-Human/Mouse Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Monoclonal Antibody (Catalog # MAB892). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: RSK1/RSK2

RSK1 (Ribosomal S6 Kinase 1), gene name RPS6KA1 (Ribosomal Protein S6 Kinase alpha 1), also known as p90RSK1 (90 kDa RSK1) or MAPKAPK 1a (MAPK Activated Protein Kinase 1a), is a widely expressed member of the RSK family of growth factor-regulated serine/threonine kinases. RSK proteins contain two non-identical kinase catalytic domains, and mediate activation of Mitogen-Activated Kinase (MAPK) cascades and stimulation of cell proliferation and differentiation. Interaction with Erk1 and Erk2 initiates an ordered phosphorylation sequence which regulates RSK activation. Phosphorylation of Ser 380 recruites PDK1 which, in turn, phosphorylates RSK1 in the activation loop at Ser 221 (or Ser227 in RSK2).

Long Name

Ribosomal Protein S6 Kinase II

Alternate Names

RSK1/RSK2