Human/Mouse PBR Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10045-100
MAB10045-SP
Detection of Human and Mouse PBR by Western Blot.
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Product Details
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Reviews (2)

Human/Mouse PBR Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human PBR in direct ELISAs. Detects human and mouse PBR in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2393B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing human PBR
Accession # P30536
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
3-25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse PBR antibody by Western Blot. View Larger

Detection of Human and Mouse PBR by Western Blot. Western blot shows lysates of SK-OV-3 human ovarian adenocarcinoma cell line, MEF mouse embryonic feeder cells, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse PBR Monoclonal Antibody (Catalog # MAB10045) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PBR at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PBR antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

PBR in HeLa Human Cell Line. PBR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse PBR Monoclonal Antibody (Catalog # MAB10045) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry PBR antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PBR in Human Prostate Cancer Tissue. PBR was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human PBR Monoclonal Antibody (Catalog # MAB10045) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm (mitochondria) in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PBR

PBR promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism. PBR is expressed at the highest levels under normal conditions in tissues that synthesize steroids.  Aberrant expression of PBR has been linked to multiple diseases, including cancer, brain injury, neurodegeneration and ischemia-reperfusion injury.

Long Name
Peripheral-type Benzodiazepine Receptor
Entrez Gene IDs
706 (Human)
Alternate Names
benzodiazepine peripheral binding site; BZRPDBI; IBP; MBRPBS; mDRC; Mitochondrial benzodiazepine receptor; PBR; PBRBPBS; PBRS; peripheral benzodiazepine receptor; Peripheral-type benzodiazepine receptor; pk18; PKBSbenzodiazapine receptor (peripheral); PTBR; translocator protein (18kDa); translocator protein; TSPO

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Reviews for Human/Mouse PBR Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse PBR Antibody
By Anonymous on 04/05/2022
Application: IHC Sample Tested: Kidney tissue Species: Mouse

Human/Mouse PBR Antibody
By Anonymous on 11/26/2021
Application: IF Sample Tested: Brain tissue Species: Mouse