Human/Mouse NRAGE Antibody Summary
Arg73-Asn254
Accession # Q9Y5V3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human, Mouse, and Rat NRAGE antibody by Western Blot. Detection of Human, Mouse, and Rat NRAGE antibody by Western Blot.](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/NRAGE_MAB38352_Western_Blot_10958.jpg)
Detection of Human, Mouse, and Rat NRAGE by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NRAGE at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
![NRAGE antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). NRAGE antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).](https://aeroglobalimagesuat.blob.core.windows.net/images/datasheets/antibody/NRAGE_MAB38352_Immunocytochemistry_11689.jpg)
NRAGE in C2C12 Mouse Cell Line. NRAGE was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NRAGE
NRAGE, also known as MAGE-D1 or Dlxin-1, is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. Human and mouse NRAGE share 87% aa identity. In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression, and facilitates NGF-dependent apoptosis.
Product Datasheets
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