Human/Mouse NRAGE Antibody

Catalog #: MAB38352 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB38352
MAB38352-SP

Detection of Human, Mouse, and Rat NRAGE by Western Blot.

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All NRAGE Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse NRAGE Antibody Summary

Specificity

Detects human NRAGE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MAGE-A4 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 613441

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NRAGE
Arg73-Asn254
Accession # Q9Y5V3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat NRAGE by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NRAGE at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

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NRAGE in C2C12 Mouse Cell Line. NRAGE was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NRAGE

NRAGE, also known as MAGE-D1 or Dlxin-1, is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. Human and mouse NRAGE share 87% aa identity. In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression, and facilitates NGF-dependent apoptosis.

Long Name

Neurotrophin Receptor-interacting MAGE Homolog

Entrez Gene IDs

9500 (Human); 94275 (Mouse); 84469 (Rat)

Alternate Names

Dlxin-1; MAGE tumor antigen CCF; MAGED1; melanoma antigen family D, 1; melanoma-associated antigen D1; Neurotrophin receptor-interacting MAGE homolog; NRAGE MAGE-D1 antigen; NRAGE