Human/Mouse NLRP3/NALP3 PE-conjugated Antibody

Catalog #: IC7578P
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC7578P

Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

2 Images

All NLRP3/NALP3 Antibodies

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse NLRP3/NALP3 PE-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse NLRP3/NALP3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human NLRP3/NALP3 is observed.

Source

Monoclonal Rat IgG_2A Clone # 768319

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse NLRP3/NALP3
Met1-Arg153
Accession # Q8R4B8

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of NLRP3/NALP3 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

View Larger

Detection of NLRP3/NALP3 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/NALP3 PE-conjugated Monoclonal Antibody (Catalog # IC7578P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of NLRP3/NALP3 antibody in Human Blood Monocytes antibody by Flow Cytometry.

View Larger

Detection of NLRP3/NALP3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human/Mouse NLRP3/NALP3 PE-conjugated Monoclonal Antibody (Catalog # IC7578P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NLRP3/NALP3

NLRP3, also known as NALP3, CIAS1, PYPAF or Cryopyrin, is a cytosolic ~120 kDa member of the NLRP family of proteins expressed in leukocytes, especially neutrophils. As a component of the inflammasome, NLRP3 activates caspases 1 and 5. Defects in NLRP3 may cause FCAS1, CINCA, or Muckle-Wells autoinflammatory syndromes. Mouse NLRP3 contains an N-terminal Pyrin domain (aa 1-91) followed by a Nacht region (aa 216-532), and seven LRRs (aa 739-988). Within the sequence used as an immunogen, mouse NLRP3 shares 78% and 93% aa identity with human and rat NLRP3, respectively. Alternate splicing of mouse NLRP3 generates additional isoforms that lack either LRR2 and 3, LRR 6 and 7, or LRR4-9.

Long Name

NLR Family, Pyrin Domain Containing 2

Entrez Gene IDs

114548 (Human); 216799 (Mouse); 287362 (Rat)

Alternate Names

AGTAVPRL; AII; AII/AVP; Angiotensin/vasopressin receptor AII/AVP-like; AVP; C1orf7; Caterpiller protein 1.1; CIAS1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cold autoinflammatory syndrome 1; Cryopyrin; Familial cold autoinflammatory syndrome; FCAS; FCU; Leucine-rich repeat-, and PYD-containing protein 3; Muckle-Wells syndrome; MWS; NACHT; NACHT, LRR and PYD containing protein 3; NACHT, LRR and PYD domains-containing protein 3; NALP3; NLR family, pyrin domain containing 3; NLRP3; Nucleotide-Binding Oligomerization Domain, Leucine Rich Repeat And Pyrin Domain Containing 3; PYPAF1; PYRIN-containing APAF1-like protein 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse NLRP3/NALP3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Differential expression of immune-regulatory proteins C5AR1, CLEC4A and NLRP3 on peripheral blood mononuclear cells in early-stage non-small cell lung cancer patients
Authors: N Pakvisal, P Kongkavito, C Sathitruan, N Pornpattan, P Boonsirika, P Ouwongpray, C Aporntewan, P Chantranuw, A Mutirangur, C Vinayanuwa
Scientific Reports, 2022-11-02;12(1):18439.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry