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Human/Mouse MSK1 Antibody

Catalog #: AF2518
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2518
AF2518-SP
Detection of Human/Mouse MSK1 by Western Blot.
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Product Details
Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse MSK1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse MSK1 in Western blots. This antibody does not detect recombinant human MSK2.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MSK1
Met1-Ala802
Accession # O75582
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse MSK1 antibody by Western Blot. View Larger

Detection of Human/Mouse MSK1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse MSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2518) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK1 was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry MSK1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

MSK1 in HeLa Human Cell Line. MSK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse MSK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2518) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSK1

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2/RSKB have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Long Name
Mitogen- and Stress-activated Protein Kinase 1
Entrez Gene IDs
9252 (Human); 73086 (Mouse)
Alternate Names
EC 2.7.11; MGC1911,90 kDa ribosomal protein S6 kinase 5; MSK1; MSK1EC 2.7.11.1; MSPK1; Nuclear mitogen- and stress-activated protein kinase 1; ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kD, polypeptide 5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; RLPK; RPS6KA5; RSKL; RSK-like protein kinase; S6K-alpha-5

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Citations for Human/Mouse MSK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. A Signaling Crosstalk Links SNAIL to the 37/67 kDa Laminin-1 Receptor Ribosomal Protein SA and Regulates the Acquisition of a Cancer Stem Cell Molecular Signature in U87 Glioblastoma Neurospheres
    Authors: L Gresseau, ME Roy, S Duhamel, B Annabi
    Cancers, 2022-11-30;14(23):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Reduction of protein kinase A-mediated phosphorylation of ATXN1-S776 in Purkinje cells delays onset of Ataxia in a SCA1 mouse model
    Authors: Judit M. Pérez Ortiz, Nissa Mollema, Nicholas Toker, Carolyn J. Adamski, Brennon O'Callaghan, Lisa Duvick et al.
    Neurobiology of Disease
  3. A Large Polysaccharide Produced by Helicobacter hepaticus Induces an Anti-inflammatory Gene Signature in Macrophages
    Authors: Camille Danne, Grigory Ryzhakov, Maria Martínez-López, Nicholas Edward Ilott, Fanny Franchini, Fiona Cuskin et al.
    Cell Host & Microbe
  4. Peptide IDR-1002 Inhibits NF-kappa B Nuclear Translocation by Inhibition of I kappa B alpha Degradation and Activates p38/ERK1/2–MSK1-Dependent CREB Phosphorylation in Macrophages Stimulated with Lipopolysaccharide
    Authors: Alejandro Huante-Mendoza, Octavio Silva-García, Javier Oviedo-Boyso, Robert E. W. Hancock, Víctor M. Baizabal-Aguirre
    Frontiers in Immunology
  5. Shigella flexneri targets the HP1 gamma subcode through the phosphothreonine lyase OspF
    Authors: Habiba Harouz, Christophe Rachez, Benoit M Meijer, Benoit Marteyn, Françoise Donnadieu, Florence Cammas et al.
    The EMBO Journal
  6. Genetic screens reveal RAS/MAPK/MSK1 modulate ataxin 1 protein levels and toxicity in SCA1
    Authors: Jeehye Park, Ismael Al-Ramahi, Qiumin Tan, Nissa Mollema, Javier R. Diaz-Garcia, Tatiana Gallego-Flores et al.
    Nature
  7. CALHM1 controls the Ca(2)(+)-dependent MEK, ERK, RSK and MSK signaling cascade in neurons.
    Authors: Dreses-Werringloer U, Vingtdeux V, Zhao H, Chandakkar P, Davies P, Marambaud P
    J Cell Sci, 2013-01-23;126(0):1199-206.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Glucocorticoid receptors recruit the CaMKIIalpha-BDNF-CREB pathways to mediate memory consolidation.
    Authors: Chen D, Bambah-Mukku D, Pollonini G, Alberini C
    Nat Neurosci, 2012-11-18;15(12):1707-14.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Ca2+ -stimulated adenylyl cyclases regulate ERK-dependent activation of MSK1 during fear conditioning.
    Authors: Sindreu CB, Scheiner ZS, Storm DR
    Neuron, 2007-01-04;53(1):79-89.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner
    Authors: M Hou, W Wang, F Hu, Y Zhang, D Yang, Q Liu
    Infect. Immun., 2018-12-19;87(1):.

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