Human/Mouse Lumican Antibody

Catalog #: MAB28461 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB28461
MAB28461-SP

Detection of Human Lumican by Western Blot.

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Human/Mouse Lumican Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Lumican in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 669401

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lumican
Gln19-Asn338
Accession # NP_002336

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Lumican by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF Membrane was probed with 2 µg/mL of Human Lumican Monoclonal Antibody (Catalog # MAB28461) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Differentially glycosylated Lumican protein species were detected between 65 and 85 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lumican

Lumican is a secreted matrix protein that belongs to the small leucine‑rich proteoglycan (SLRP) class II family. Mature Lumican has a negatively charged N‑terminal domain that contains tyrosine sulfates, followed by twelve leucine‑rich repeats, which are flanked by cysteine loops. Lumican is expressed widely in mammalian connective tissues, while corneal Lumican exists as a keratan sulfate proteoglycan in adult cartilage, Lumican exists primarily as a glycoprotein and lacks keratan sulfate. The amino acid sequence of human Lumican shares 88% amino acid sequence identity with that of mature mouse Lumican.

Entrez Gene IDs

4060 (Human); 17022 (Mouse)

Alternate Names

KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican