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Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody

Catalog #: IC2191P
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC2191P
Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry.
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Citations (8)
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Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Source
Monoclonal Mouse IgG1 Clone # 27537
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-12/IL-35 p35 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PE-conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of IL-12/IL-35 p35 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PE-conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-12/IL-35 p35

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

References
  1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
  2. Collison, L.W. et al. (2007) Nature 450:566.
  3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
  4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
Long Name
Interleukin 12 p35
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names
CLMF p35; CLMF; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12, subunit p35; IL-12/IL-35 p35; IL-12A; IL35 subunit; IL35p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; p35

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Citations for Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The effect of human placental chorionic villi derived mesenchymal stem cell on triple-negative breast cancer hallmarks
    Authors: AT Alshareeda, E Rakha, A Alghwainem, B Alrfaei, B Alsowayan, A Albugami, AM Alsubayyil, M Abomraee, NK Mohd Zin
    PLoS ONE, 2018-11-20;13(11):e0207593.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Modulation of the Tumor Microenvironment by CXCR4 Antagonist-Armed Viral Oncotherapy Enhances the Antitumor Efficacy of Dendritic Cell Vaccines against Neuroblastoma in Syngeneic Mice
    Authors: M Komorowski, J Tisonczyk, A Kolakowska, R Drozdz, D Kozbor
    Viruses, 2018-08-26;10(9):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Dysregulated ICOS+ proinflammatory and suppressive regulatory T cells�in patients with rheumatoid arthritis
    Authors: Hong‑Xia Wang, Xia Kang, Shuai Chu, Haixia Li, Xin Li, Xiaofeng Yin et al.
    Experimental and Therapeutic Medicine
  4. Reprogramming antitumor immunity against chemoresistant ovarian cancer by a CXCR4 antagonist-armed viral oncotherapy
    Authors: Marcin P Komorowski
    Mol Ther Oncolytics, 2016-12-14;3(0):16034.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Generation of inducible immortalized dendritic cells with proper immune function in vitro and in vivo.
    Authors: Richter C, Thieme S, Bandola J, Laugsch M, Anastassiadis K, Brenner S
    PLoS ONE, 2013-04-23;8(4):e62621.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Myeloid dendritic cells (DCs) of mice susceptible to paracoccidioidomycosis suppress T cell responses whereas myeloid and plasmacytoid DCs from resistant mice induce effector and regulatory T cells.
    Authors: Pina A, de Araujo E, Felonato M, Loures F, Feriotti C, Bernardino S, Barbuto J, Calich V
    Infect Immun, 2013-01-22;81(4):1064-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. IL-35 over-expression increases apoptosis sensitivity and suppresses cell growth in human cancer cells.
    Biochem Biophys Res Commun, 2012-11-12;430(1):364-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Cutting edge: Human regulatory T cells require IL-35 to mediate suppression and infectious tolerance.
    Authors: Chaturvedi V, Collison LW, Guy CS, Workman CJ, Vignali DA
    J. Immunol., 2011-05-16;186(12):6661-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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