Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody

Catalog #: IC1935C Datasheet / COA / SDS

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IC1935C

Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.

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Human/Mouse HIF-1 alpha/HIF1A PerCP-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Recognizes human and mouse HIF-1 alpha in direct ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 241812

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

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Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl₂was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

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Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with 1 mM DFO overnight was stained with Mouse Anti-Human/Mouse HIF-1a PerCP-conjugated Monoclonal Antibody (Catalog # IC1935C, filled histogram) or isotype control antibody (IC002C, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with Flow Cytometry FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name

Hypoxia Inducible Factor 1 Subunit Alpha

Entrez Gene IDs

3091 (Human); 15251 (Mouse); 29560 (Rat)

Alternate Names

AINT; anti-HIF-1 alpha; anti-HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1; HIF1A; HIF-1a; HIF-1alpha; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8