Human/Mouse CXCR7/RDC-1 APC-conjugated Antibody

Catalog #: FAB4227A
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB4227A-025
FAB4227A-100

Detection of CXCR7/RDC‑1 in MCF‑7 Human Cell Line by Flow Cytometry.

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All CXCR7/RDC-1 Antibodies

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Citations (7)

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Human/Mouse CXCR7/RDC-1 APC-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human CXCR7/RDC-1 in flow cytometry of five distinct human CXCR7 transfectants, but not their respective parental lines.

Source

Monoclonal Mouse IgG₁ Clone # 11G8

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CXCR7 encoding plasmid
Accession # P25106

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CXCR7/RDC‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human/Mouse CXCR7/RDC-1 APC-conjugated Mono-clonal Antibody (Catalog # FAB4227A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR7/RDC-1

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I‑TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96%, and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) (J. Biol. Chem. 2005, 280(42):35760, J. Immunol. 2006, 176(4):2197) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (J. Exp. Med. 2006, 203(9):2201). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

Entrez Gene IDs

57007 (Human); 12778 (Mouse); 84348 (Rat)

Alternate Names

ACKR3; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7; CMKOR1; CXCR7; CXC-R7; CXCR-7; G protein-coupled receptor; GPR159; GPR159G-protein coupled receptor 159; RDC-1; RDC1G-protein coupled receptor RDC1 homolog

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human/Mouse CXCR7/RDC-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Breast Cancer: An Examination of the Potential of ACKR3 to Modify the Response of CXCR4 to CXCL12
Authors: I Del Molino, GC Wilkins, A Meeson, S Ali, JA Kirby
Int J Mol Sci, 2018-11-14;19(11):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
Authors: E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá
Arthritis Res. Ther., 2018-06-07;20(1):114.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Mutational Analysis of Atypical Chemokine Receptor 3 (ACKR3/CXCR7) Interaction with its Chemokine Ligands CXCL11 and CXCL12
Authors: Nikolaus Heveker
J. Biol. Chem., 2016-11-14;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Downregulation of CXCL12 in mesenchymal stromal cells by TGF? promotes breast cancer metastasis
Oncogene, 2016-09-26;0(0):.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
CXCR4 expression on pathogenic T cells facilitates their bone marrow infiltration in a mouse model of aplastic anemia.
Authors: Arieta Kuksin C, Gonzalez-Perez G, Minter L
Blood, 2015-02-03;125(13):2087-94.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Llama-derived single variable domains (nanobodies) directed against chemokine receptor CXCR7 reduce head and neck cancer cell growth in vivo.
Authors: Maussang D, Mujic-Delic A, Descamps F, Stortelers C, Vanlandschoot P, Stigter-van Walsum M, Vischer H, van Roy M, Vosjan M, Gonzalez-Pajuelo M, van Dongen G, Merchiers P, van Rompaey P, Smit M
J Biol Chem, 2013-08-26;288(41):29562-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CXCR7 protein is not expressed on human or mouse leukocytes.
Authors: Berahovich RD, Zabel BA, Penfold ME, Lewen S, Wang Y, Miao Z, Gan L, Pereda J, Dias J, Slukvin II, McGrath KE, Jaen JC, Schall TJ
J. Immunol., 2010-10-01;185(9):5130-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry