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Human/Mouse CRY1 Antibody

Catalog #: AF3764
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3764
AF3764-SP
CRY1 in MOLT‑4 Human Cell Line.
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Citations (1)
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Human/Mouse CRY1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CRY1 in direct ELISAs and Western Blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CRY1
Lys115-His224
Accession # Q16526
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CRY1
Recombinant Mouse CRY1
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse testis
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CRY1 antibody in MOLT-4 Human Cell Line by Immunocytochemistry (ICC). View Larger

CRY1 in MOLT‑4 Human Cell Line. CRY1 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Goat Anti-Human/Mouse CRY1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3764) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRY1

CRY1 is a critical component of the circadian oscillator that represses CLOCK:BMAL1 mediated transcription when translocated into the nucleus. It is a member of the DNA photolyase family but has no photolyase activity. Over the region used as immunogen, human and mouse CRY1 share 94% amino acid sequence homology.

Long Name
Cryptochrome 1
Entrez Gene IDs
1407 (Human); 12952 (Mouse); 299691 (Rat)
Alternate Names
CRY1; cryptochrome 1 (photolyase-like); Cryptochrome I; PHLL1; PHLL1cryptochrome-1; photolyase-like cryptochrome 1

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Citation for Human/Mouse CRY1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Multiplexed CRISPR-Cas9 system in a single adeno-associated virus to simultaneously knock out redundant clock genes
    Authors: B Kim, J Kim, M Chun, I Park, D Kwak, M Choi, K Kim, HK Choe
    Scientific Reports, 2021-01-28;11(1):2575.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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