Human/Mouse Bcl-x Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse Bcl‑x by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104cells, lane 1 and 2.5 x 104cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Immunoprecipitation of Human Bcl-x. Bcl-x was immunoprecipitated from lysates (7 x 105cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30‑60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.
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Detection of Human and Mouse Bcl‑x by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl‑x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl‑x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: Bcl-x
The peptide corresponds to amino acids 49-68 of human and mouse, long and short forms of Bcl-x. The sequence used for immunization differs significantly from the sequences of the corresponding region of other members of the Bcl-2 family. Cysteine was added to the amino-terminal for coupling to KLH and for coupling to an affinity matrix.
Product Datasheets
Citations for Human/Mouse Bcl-x Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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The Apoptotic Effect of Caffeic or Chlorogenic Acid on the C32 Cells That Have Simultaneously Been Exposed to a Static Magnetic Field
Authors: M Kimsa-Dude, A Synowiec-W, A Krawczyk, A Kosowska, M Kimsa-Furd, T Francuz
International Journal of Molecular Sciences, 2022-03-31;23(7):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Carbon ion triggered immunogenic necroptosis of nasopharyngeal carcinoma cells involving necroptotic inhibitor BCL-x
Authors: C Bao, Y Sun, B Dwarakanat, Y Dong, Y Huang, X Wu, C Guha, L Kong, JJ Lu
Journal of Cancer, 2021-01-01;12(5):1520-1530.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint.
Authors: Schmitt E, Beauchemin M, Bertrand R
Oncogene, 2007-03-19;26(40):5851-65.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.
Authors: Armstrong T, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP
Clin. Cancer Res., 2004-11-01;10(21):7427-37.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.
Authors: Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
Clin. Cancer Res., 2004-03-15;10(6):2100-8.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.
Authors: Raman VS, Akondy RS, Rath S, Bal V, George A
J. Immunol., 2003-12-01;171(11):5876-81.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
Authors: Sade H, Krishna S, Sarin A
J. Biol. Chem., 2003-10-28;279(4):2937-44.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.
Authors: Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
J. Exp. Med., 2002-07-01;196(1):87-95.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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