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Human/Mouse BACE-1 Ectodomain Antibody

Catalog #: AF931
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF931
AF931-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse BACE-1 Ectodomain Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse BACE‑1 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) ADAM8, recombinant mouse ADAM9, rhADAM15, and rhTACE is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BACE‑1 Ectodomain
Thr22-Tyr460
Accession # P56817
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human BACE‑1 (Catalog # 931‑AS)
Recombinant Mouse BACE‑1 (Catalog # 2976‑AS)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human BACE‑1 (Catalog # 931‑AS) or Recombinant Mouse BACE‑1 (Catalog # 2976-AS), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BACE-1

BACE-1 is an aspartic protease and an integral membrane protein (1 - 5). BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein beta site in the brain to generate the amyloid beta peptide. Because the amyloid beta peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5 - 5.5), consistent with its proposed function in an acidic intracellular compartment.

References
  1. Ermolieff, J. et al. (2000) Biochemistry 39:12450.
  2. Lin, X. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1456.
  3. Sinha, S. et al. (1999) Nature 402:537.
  4. Vassar, R. et al. (1999) Science 286:735.
  5. Yan, R. et al. (1999) Nature 402:533.
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 1
Entrez Gene IDs
23621 (Human); 23821 (Mouse)
Alternate Names
APP beta-secretase; ASP2; Aspartyl protease 2; BACE1; BACE-1; BACEAsp 2; beta-secretase 1 precursor variant 1; beta-secretase 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; beta-site APP-cleaving enzyme 1; beta-site APP-cleaving enzyme; EC 3.4.23; EC 3.4.23.46; FLJ90568; HSPC104; KIAA1149; memapsin-2; Membrane-associated aspartic protease 2; transmembrane aspartic proteinase Asp2

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Citations for Human/Mouse BACE-1 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Development of a specific ELISA to measure BACE1 levels in human tissues
    Authors: Amanda Gonzales, Boris Decourt, Aaron Walker, Rachel Condjella, Hikmet Nural, Marwan N. Sabbagh
    Journal of Neuroscience Methods
  2. n-3 Polyunsaturated fatty acids modulate carcinogen-directed non-coding microRNA signatures in rat colon.
    Authors: Davidson LA, Wang N, Shah MS, Lupton JR, Ivanov I, Chapkin RS
    Carcinogenesis, 2009-12-01;30(12):2077-84.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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