Human/Mouse Acetyl Histone H4 Antibody

Catalog #: AF5215
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5215
AF5215-SP

Detection of Human Acetyl Histone H4 by Western Blot.

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All Histone H4 Antibodies

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Acetyl Histone H4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects immunogen in direct ELISAs and human Acetyl Histone H4 in Western blots. Reactivity with non-acetylated peptide was removed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Tetrahymena pyriformis acetylated Histone H4 synthetic peptide
AGGKGGKGMGKVGAKRHSK
Accession # P02310

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Acetyl Histone H4 by Western Blot. Western blot shows lysates of Tera-2 human embryonic lung carcinoma cell line, Nalm-6 human Pre-B acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Acetyl Histone H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5215) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Acetyl Histone H4 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

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Histone H4 in NTera‑2 Human Cell Line. Histone H4 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Acetyl Histone H4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5215) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histone H4

Histone 4 (H4/HIST1H4I; also H4M and HIST4H4) is a 12-15 kDa member of the histone H4 family of molecules. It is ubiquitously expressed, with H4 initially synthesized and deposited in replicating chromatin during S phase. H4 undergoes multiple posttranslational modifications. At the G2/M transition, H4 is monomethylated on Lys20 and possibly contributes to transcription initiation/elongation. As the cell moves into the M/G1 phase, Lys20 becomes either dimethylated or trimethylated, contributing to DNA repair and telomere maintenance, respectively. H4 is also known to be phosphorylated on Ser2 and 48, plus Tyr52 and 89, and acetylated on at least nine sites. Human histone 4 is 103 amino acids (aa) in length. Full length histone 4 is identical in aa sequence to mouse histone 4, and 78% aa identical to Tetrahymena histone 4 over aa 2-20 used for immunization.

Entrez Gene IDs

121504 (Human); 319159 (Mouse)

Alternate Names

H4; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4/p; H4F2; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; histone 4, H4; Histone Cluster 1 H4; Histone Cluster 1 H4i; Histone Cluster 4 H4; histone cluster 4, H4; Histone H4; MGC24116

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Acetyl Histone H4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Neutrophil extracellular traps induce IL-1 beta production by macrophages in combination with lipopolysaccharide
Authors: Zhongshuang Hu, Taisuke Murakami, Hiroshi Tamura, Johannes Reich, Kyoko Kuwahara-Arai, Toshiaki Iba et al.
International Journal of Molecular Medicine
Dissecting mammalian spermatogenesis using spatial transcriptomics
Authors: H Chen, E Murray, A Sinha, A Laumas, J Li, D Lesman, X Nie, J Hotaling, J Guo, BR Cairns, EZ Macosko, CY Cheng, F Chen
Cell Reports, 2021-11-02;37(5):109915.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Decreased KAT5 Expression Impairs DNA Repair and Induces Altered DNA Methylation in Kidney Podocytes
Authors: A Hishikawa, K Hayashi, T Abe, M Kaneko, H Yokoi, T Azegami, M Nakamura, N Yoshimoto, T Kanda, Y Sakamaki, H Itoh
Cell Rep, 2019-01-29;26(5):1318-1332.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
SIRT3-mediated inhibition of FOS through histone H3 deacetylation prevents cardiac fibrosis and inflammation
Authors: Xavier Palomer, M. Silvia Román-Azcona, Javier Pizarro-Delgado, Ana Planavila, Francesc Villarroya, Brenda Valenzuela-Alcaraz et al.
Signal Transduction and Targeted Therapy
KAT6A regulates stemness of aging bone marrow-derived mesenchymal stem cells through Nrf2/ARE signaling pathway
Authors: Dongdong Fei, Yazheng Wang, Qiming Zhai, Xige Zhang, Yang Zhang, Yang Wang et al.
Stem Cell Research & Therapy
Environmental Enrichment Modified Epigenetic Mechanisms in SAMP8 Mouse Hippocampus by Reducing Oxidative Stress and Inflammaging and Achieving Neuroprotection
Authors: Christian Griñan-Ferré, Dolors Puigoriol-Illamola, Verónica Palomera-Ávalos, David Pérez-Cáceres, Júlia Companys-Alemany, Antonio Camins et al.
Frontiers in Aging Neuroscience