Human MMP-17 Antibody

Catalog #: MAB7796 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7796
MAB7796-SP

Neutralization of Human MMP‑17 by Human MMP-17 Antibody.

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All MMP-17 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-17 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MMP-17 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MMP-24 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 823511

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-17
Pro42-Ala534
Accession # Q9ULZ9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by itsability to neutralize Recombinant Human MMP-17 (1.0 μg/mL, Catalog # 7796-MP) cleavage of the fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH₂ (10μM, Catalog # ES003). The Neutralization Dose (ND₅₀) is < 9.0 μg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Neutralization of Human MMP‑17 by Human MMP-17 Antibody. The cleavage of Mca-PLAQAV-Dpa-RSSSR-NH₂(10 µM, ES003) by Recombinant Human MMP-17 (1.0 ug/mL, 7796-MP) is measured after it has been preincubated with increasing concentrations of Mouse Anti-Human MMP-17 Monoclonal Antibody (Catalog # MAB7796). The ND₅₀ is typically <9.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-17

Matrix metalloproteinase 17 (MMP‑17) is a zinc metalloproteinase that is an integral membrane protein (1). It belongs to the membrane‑type MMP (MT‑MMP) subfamily, and is also known as MT4‑MMP. It is associated with the cell membrane by a glycosylphosphatidylinositol (GPI) anchor (1). It has the potential to function as a pro‑tumor necrosis factor‑ alpha (TNF‑ alpha ) convertase (2). Unlike MMP‑14 (MT1‑MMP), it is a poor activator of pro‑MMP‑2. MMP‑17 is relatively poor at digesting components of the extracellular matrix but does cleave fibrinogen and fibrin (2). MMP‑17 is known to activate the aggrecanase ADAMTS‑4 through proteolysis (3). MMP‑17 is expressed in many tissues with the highest levels seen in the brain, colon, ovary, testis, and kidney papilla (4). It has been detected in several human cancers, including gliomas, prostate carcinomas, and breast carcinomas (5).

References

Itoh, Y. et al. (1999) J. Biol. Chem. 274:34260.
English, W. R. et al. (2000) J. Biol. Chem. 275:14046.
Gao, G. et al. (2004) J. Biol. Chem. 279:10042.
Srichai, M. B. et al. (2011) PLoS ONE 6:e17099.
Sohail, A. et al. (2008) Cancer Metastasis Rev. 27:289.

Long Name

Matrix Metalloproteinase 17

Entrez Gene IDs

4326 (Human); 23948 (Mouse); 288626 (Rat)

Alternate Names

matrix metallopeptidase 17 (membrane-inserted); matrix metalloproteinase 17 (membrane-inserted); matrix metalloproteinase-17; membrane-type matrix metalloproteinase 4; membrane-type-4 matrix metalloproteinase; MMP17; MMP-17; MT4MMP; MT4-MMP; MT-MMP 4; MTMMP4