Human MKK7 Antibody

Catalog #: AF3579 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3579
AF3579-SP

Detection of Human MKK7 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MKK7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MKK7 in Western blots. Does not cross-react with other recombinant human mitogen-activated protein kinase kinases.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MKK7
Met1-Arg419
Accession # O14733

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human tonsil and placenta

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human MKK7 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, SW480 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human MKK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3579) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MKK7 was detected at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

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Detection of Human MKK7 by Western Blot. Western blot shows recombinant human MEKs and recombinant human MKKs Catalog#s XXX (XXX ng/lane). PVDF membrane was probed with 2 µg/mL Goat Anti-Human MKK7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3579) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for MKK7 was detected at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKK7

Mitogen-activated protein kinase kinase 7 (MKK7 or MAP2K7), also known as MEK7 and Jun kinase kinase 2 (JNKK2), is activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation at Ser271 and Thr275 by several upstream MAPK kinase kinases (MAP3Ks). MKK7 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr.

Long Name

Mitogen-activated Protein Kinase Kinase 7

Entrez Gene IDs

5609 (Human); 26400 (Mouse); 363855 (Rat)

Alternate Names

c-Jun N-terminal kinase kinase 2; dual specificity mitogen-activated protein kinase kinase 7; EC 2.7.12.2; JNK kinase 2; JNK-activating kinase 2; JNKK 2; JNKK2; MAP kinase kinase 7; MAP2K7; MAPK/ERK kinase 7; MAPKK7; MEK 7; MEK7; mitogen-activated protein kinase kinase 7; MKE7; MKK7; MKK7MAPKK 7; PRKMK7