Human Mimecan Biotinylated Antibody

Catalog #: BAF2660 Datasheet / COA / SDS

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BAF2660

All Mimecan Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mimecan Biotinylated Antibody Summary

Specificity

Detects human Mimecan in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant mouse Mimecan is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mimecan
Ala20-Phe298
Accession # P20774

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Mimecan

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mimecan

Mimecan, also known as KSPG25 (25 kDa Keratan Sulfate Proteoglycan) and Osteoglycin, is an extracellular matrix protein belonging to the class III subfamily of the small leucine rich proteoglycan (SLRP) family. SLRPs are characterized by leucine‑rich repeats in the central domain, which is flanked by N- and C‑terminal cysteine‑rich domains. The core proteins contain N-linked oligosaccharides as well as glycosaminoglycan side chains. Mimecan is synthesized as a preproprotein that is processed to generate proteins of different sizes including Osteoglycin (containing 93 most C‑terminal amino acid residues) and Mimecan (containing 223 most C‑terminal residues). Human Mimecan shares approximately 85% amino acid sequence homology with the mouse or rat Mimecan.

Entrez Gene IDs

4969 (Human); 18295 (Mouse)

Alternate Names

DKFZp586P2421; KSPG25; mimecan proteoglycan; Mimecan; OGN; OIF; OIFOG; osteoglycin (osteoinductive factor); osteoglycin; Osteoinductive factor; SLRR3A; SLRR3Acorneal keratan sulfate proteoglycan