Human Melanocortin-5 R/MC5R Antibody

Catalog #: MAB8205
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8205
MAB8205-SP

Detection of Melanocortin-5 R/MC5R in HEK293 Human Cell Line Transfected with Human Melanocortin-5 R/MC5R and eGFP by Flow Cytometry.

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Citations (2)

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Human Melanocortin-5 R/MC5R Antibody Summary

Species Reactivity

Human

Specificity

Stains human Melanocortin-5 R/MC5R transfected cells but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2A Clone # 821012

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human Melanocortin-5 R/MC5R
Met1-Asp325
Accession # P33032

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

Detection of Melanocortin-5 R/MC5R antibody in HEK293 Human Cell Line Transfected with Human Melanocortin-5 R/MC5R and eGFP antibody by Flow Cytometry.

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Detection of Melanocortin-5 R/MC5R in HEK293 Human Cell Line Transfected with Human Melanocortin-5 R/MC5R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Melanocortin-5 R/MC5R and eGFP was stained with and either (A) Mouse Anti-Human Melanocortin-5 R/MC5R Monoclonal Antibody (Catalog # MAB8205) or (B) Mouse IgG_2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melanocortin-5 R/MC5R

Melanocortin 5R (MC5R) is a member of the melanocortin receptor family. The 325 amino acid G-protein-coupled 7-transmembrane glycoprotein is expressed in skeletal muscle, where engagement by a-melanocyte stimulating hormone increases glucose uptake and stimulates a thermogenic response. Expression is also found in sebaceous glands and other skin structures, and on selected hematopoietic cell lines. Human MC5R shares 80-81% amino acid sequence identity with mouse and rat MC5R.

Long Name

Melanocortin 5 Receptor

Entrez Gene IDs

4161 (Human); 17203 (Mouse); 25726 (Rat)

Alternate Names

MC2; MC-2; MC5; MC5R; MC5-R; melanocortin 5 receptor; melanocortin receptor 5; Melanocortin-5 R; melanocortin-5 receptor; Melanocortin5R; Melanocortin-5R

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human Melanocortin-5 R/MC5R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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