Human Mcl-1 Antibody

Name: Human Mcl-1 Antibody
Brand: R&D Systems
SKU: MAB828
Rating: 4 (1 reviews)

Catalog #: MAB828
3 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB828
MAB828-SP

Detection of Human Mcl‑1 by Western Blot.

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Product Details

Citations (3)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mcl-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mcl-1.

Source

Monoclonal Mouse IgG_2B Clone # 542808

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Mcl-1
Met1-Gly230
Accession # Q07820

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human lymphoma

Knockout Validated

Mcl‑1 is specifically detected in A431 human epithelial carcinoma parental cell line but is not detectable in Mcl‑1 knockout A431 cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Mcl‑1 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, THP-1 human acute monocytic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Mcl-1 Monoclonal Antibody (Catalog # MAB828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Mcl-1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Knockout Validated

Western Blot Shows Human Mcl-1 Antibody Specificity by Using Knockout Cell Line.

View Larger

Western Blot Shows Human Mcl‑1 Specificity by Using Knockout Cell Line. Western blot shows lysates of A431 human epithelial carcinoma parental cell line and Mcl-1 knockout A431 cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Mcl-1 Monoclonal Antibody (Catalog # MAB828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Mcl-1 at approximately 40 kDa (as indicated) in the parental A431 cell line, but is not detectable in knockout A431 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mcl-1

Mcl-1 (myeloid cell leukemia-1; also known as Bcl-2-like protein 3) is a member of the Bcl-2 family of proteins. Alternative splicing creates two distinct isoforms: 40 kDa Mcl-1L (long; 350 amino acids (aa)) enhances cell survival by inhibiting apoptosis, while 32 kDa Mcl-1S (short; 271 aa with divergence in the last 41 aa) promotes apoptosis. The elimination of Mcl-1L is a required step for DNA damage-induced apoptosis. Mcl-1 can be modified by phosphorylation on S121 and T163 by JNK, which triggers apoptosis, or polyubiquitination, which enhances degradation of Mcl-1. Within the first 230 aa, human Mcl-1 shares ~68% aa identity with mouse and rat Mcl-1.

Long Name

Myeloid Cell Leukemia Sequence 1

Entrez Gene IDs

4170 (Human); 17210 (Mouse)

Alternate Names

BCL2L3; bcl2-L-3; BCL2L3MGC104264; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; EAT; induced myeloid leukemia cell differentiation protein Mcl-1; Mcl1; Mcl-1; mcl1/EAT; MCL1-ES; MCL1L; MCL1S; MGC1839; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related); TM

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Mcl-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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p53-mediated heterochromatin reorganization regulates its cell fate decisions
Authors: Sathish Kumar Mungamuri, Erica Kay Benson, Shaomeng Wang, Wei Gu, Sam W Lee, Stuart A Aaronson
Nature Structural & Molecular Biology
High-Complexity shRNA Libraries and PI3 Kinase Inhibition in Cancer: High-Fidelity Synthetic Lethality Predictions
Authors: M Mues, L Karra, D Romero-Moy, A Wandler, MJ Hangauer, O Ksionda, Y Thus, M Lindenberg, K Shannon, MT McManus, JP Roose
Cell Rep, 2019-04-09;27(2):631-647.e5.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Targeting BET Proteins With a PROTAC Molecule Elicits Potent Anticancer Activity in HCC Cells
Authors: Huapeng Zhang, Gongquan Li, Yi Zhang, Jihua Shi, Bing Yan, Hongwei Tang et al.
Frontiers in Oncology